Cordonnier A, Rivière Y, Montagnier L, Emerman M
Unité d'Oncologie Virale (Centre National de la Recherche Scientifique, UA1157), Institut Pasteur, Paris, France.
J Virol. 1989 Oct;63(10):4464-8. doi: 10.1128/JVI.63.10.4464-4468.1989.
Sequence comparison of the human immunodeficiency virus type 1 and type 2 env genes revealed the presence of six linear regions in the extracellular glycoprotein that are highly conserved. To investigate the functional significance of these regions, we made short deletions in each and assayed the ability of the mutated proteins to bind CD4 antigen. Small deletions in four of the highly conserved regions drastically reduced receptor binding. Some deletions interfered with the maturation of the envelope glycoprotein, but maturation did not necessarily correlate with the ability to bind CD4 antigen.
1型和2型人类免疫缺陷病毒包膜基因的序列比较显示，细胞外糖蛋白中存在六个高度保守的线性区域。为了研究这些区域的功能意义，我们对每个区域进行了短片段缺失，并检测了突变蛋白结合CD4抗原的能力。四个高度保守区域中的小片段缺失显著降低了受体结合能力。一些缺失干扰了包膜糖蛋白的成熟，但成熟并不一定与结合CD4抗原的能力相关。
