Langton P, Ward S M, Carl A, Norell M A, Sanders K M
Department of Physiology, University of Nevada School of Medicine, Reno 89511.
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7280-4. doi: 10.1073/pnas.86.18.7280.
Interstitial cells of Cajal (ICC) have been suggested as pacemaker cells in the gastrointestinal tract. A method was developed to isolate ICC from the slow-wave pacemaker region of the canine proximal colon. These cells were identified under phase-contrast microscopy, and their identity was verified by comparing their ultrastructure with the morphology of ICC in situ. Patch-clamp experiments demonstrated that these cells are excitable; voltage-dependent inward and outward currents were elicited by depolarization. Inward current transients were identified as calcium currents. A portion of the outward current appears to be due to Ca2+-activated K channels commonly expressed in these cells. ICC were also spontaneously active, generating electrical depolarizations similar in waveform to slow-wave events of intact colonic muscles. These findings are consistent with the hypothesis that ICC initiate rhythmicity in the colon.
Cajal间质细胞（ICC）被认为是胃肠道的起搏细胞。已开发出一种从犬近端结肠慢波起搏区域分离ICC的方法。这些细胞在相差显微镜下被识别，并通过将其超微结构与原位ICC的形态进行比较来验证其身份。膜片钳实验表明这些细胞是可兴奋的；去极化可引发电压依赖性内向和外向电流。内向电流瞬变被确定为钙电流。一部分外向电流似乎是由于这些细胞中普遍表达的Ca2+激活钾通道所致。ICC也具有自发活性，产生波形与完整结肠肌肉慢波事件相似的电去极化。这些发现与ICC启动结肠节律性的假设一致。
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