Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Sci Rep. 2018 Sep 10;8(1):13544. doi: 10.1038/s41598-018-31992-4.
Oriented smooth muscle layers in the intestine contract rhythmically due to the action of interstitial cells of Cajal (ICC) that serve as pacemakers of the intestine. Disruption of ICC networks has been reported in various intestinal motility disorders, which limit the quality and expectancy of life. A significant challenge in intestinal smooth muscle engineering is the rapid loss of function in cultured ICC and smooth muscle cells (SMC). Here we demonstrate a novel approach to maintain the function of both ICC and SMC in vitro. Primary intestinal SMC mixtures cultured on feeder cells seeded electrospun poly(3-caprolactone) scaffolds exhibited rhythmic contractions with directionality for over 10 weeks in vitro. The simplicity of this system should allow for wide usage in research on intestinal motility disorders and tissue engineering, and may prove to be a versatile platform for generating other types of functional SMC in vitro.
肠道中的平滑肌层由于间质细胞 Cajal（ICC）的作用而有节奏地收缩，ICC 作为肠道的起搏器。在各种肠道动力障碍中，ICC 网络的破坏已被报道，这限制了生活的质量和预期寿命。肠道平滑肌工程的一个重大挑战是培养的 ICC 和平滑肌细胞（SMC）功能迅速丧失。在这里，我们展示了一种在体外维持 ICC 和 SMC 功能的新方法。在饲养细胞上培养的原代肠道 SMC 混合物在电纺聚己内酯支架上表现出具有方向性的有节奏收缩，在体外超过 10 周。该系统的简单性应该允许在肠道动力障碍和组织工程的研究中广泛使用，并且可能被证明是在体外生成其他类型功能性 SMC 的多功能平台。
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