Thabet Mahmoud, Hemida Reda, Hasan Mohammad, Elshamy Maged, Elfaraash Mohammad, Emam Mohammad
J Exp Ther Oncol. 2014;10(4):247-53.
Cervical cancer remains a significant problem worldwide particularly in underdeveloped countries. It is necessary to have a persistent infection of the cervix with a high-risk or oncogenic human Papillomavirus (HPV) virus to develop cervical cancer.
To study the association between HPV and pre-invasive and invasive cancer cervix among patients referred to Early Cancer Detection Clinic of Obstetrics and Gynecology Department, Mansoura University Hospital, Delta region, Egypt.
Cervical specimens of 100 histologically confirmed premalignant and malignant cervical lesions were subjected to HPV detection and genotyping by extraction of DNA from cervical biopsy using a commercial PCR kit.
HPV DNA testing was done, 36 cases were positive (36%). Correlations of age, duration of marriage, and parity were non significant (P = 0.56, 0.72, and 0.35 respectively) while correlations of residence, oral contraceptive use, smoking, and immunosuppresion were sig- nificant (P = 0.006, 0.001, 0.001, and 0.01 respectively). The prevalence of HPV in premalignant and malignant cervical lesions in our study was 39.5% & 33.3% respec tively. The commonest HPV genotypes associated with premalignant cervical lesions were HPV16; 11/17(64.7%) and HPV18; 11/17 (64.7%) mostly in the form of mul- tiple infections with HPV16+18; 7/17 (41.17%). The commonest HPV genotypes associated with malignant cervical lesions in our cases were HPV16; 15/19 (78.9%) and HPV18; 13/19 (68.42%) also in the form of multiple infections with HPV16+18; 10/19 (52.63%).
The prevalence of HPV in premalignant and malignant cervical lesions was 39.5% & 33.3% respectively, this means that HPV is not the main cause of premalignant and malignant cervical lesions in Delta region in Egypt. HPV infection mostly in the form of multiple infections with HPV16+18 genotypes. Further studies are needed to clarify actual association of HPV and premalignant and malignant cervical lesions to determine the usefulness of HPV vaccination in our locality.
宫颈癌在全球范围内仍是一个重大问题，尤其是在欠发达国家。宫颈癌的发生需要宫颈持续感染高危型或致癌型人乳头瘤病毒（HPV）。
研究在埃及三角洲地区曼苏拉大学医院妇产科早期癌症检测诊所就诊的患者中，HPV与宫颈浸润前病变及浸润性癌之间的关联。
使用商业PCR试剂盒从宫颈活检组织中提取DNA，对100例经组织学确诊的宫颈恶性前病变和恶性病变的宫颈标本进行HPV检测和基因分型。
进行了HPV DNA检测，36例呈阳性（36%）。年龄、婚姻持续时间和产次之间的相关性不显著（P值分别为0.56、0.72和0.35），而居住地、口服避孕药使用、吸烟和免疫抑制之间的相关性显著（P值分别为0.006、0.001、0.001和0.01）。在我们的研究中，宫颈恶性前病变和恶性病变中HPV的患病率分别为39.5%和33.3%。与宫颈恶性前病变相关的最常见HPV基因型是HPV16；11/17（64.7%）和HPV18；11/17（64.7%），大多为HPV16 + 18多重感染形式；7/17（41.17%）。在我们的病例中，与宫颈恶性病变相关的最常见HPV基因型是HPV16；15/19（78.9%）和HPV18；13/19（68.42%），同样为HPV16 + 18多重感染形式；10/19（52.63%）。
宫颈恶性前病变和恶性病变中HPV的患病率分别为39.5%和33.3%，这意味着在埃及三角洲地区，HPV不是宫颈恶性前病变和恶性病变的主要原因。HPV感染大多为HPV16 + 18基因型的多重感染形式。需要进一步研究以阐明HPV与宫颈恶性前病变和恶性病变之间的实际关联，从而确定HPV疫苗接种在我们当地的有效性。
