State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
Sichuan New Lotus Traditional Chinese Herb Limited Company, Chengdu, China.
J Ethnopharmacol. 2015 Feb 23;161:92-8. doi: 10.1016/j.jep.2014.12.013. Epub 2014 Dec 12.
Penthorum chinense Pursh (Penthoraceae) has been used as a Miao ethnomedicine for the treatment of jaundice, cholecystitis, edema, infectious hepatitis and anti-drunk hangover in China. The aim of present study is to investigate the possible protective effects of Penthorum chinense against chronic ethanol-induced liver injury.
Mice were fed a Lieber-DeCarli liquid diet containing alcohol or isocaloric maltose dextrin as control diet with or without aqueous extract of Penthorum chinense (PCP, 5.15 and 10.30 g/kg/BW) for 4 weeks. Silymarin (86 mg/kg) was used as positive control to compare the efficacy of PCP against chronic ethanol-induced hepatotoxicity.
Treatment with PCP (10.30 g/kg) significantly reduced the increases in serum ALT and AST levels, hepatic lipid accumulation and inflammatory cytokines (i.e. TNF-α, IL-6), which were induced by chronic ethanol exposure. PCP was also found to attenuate reactive oxygen species (ROS) generation and malondialdehyde (MDA) level, restore the glutathione (GSH) depletion, and increase the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. In addition, PCP supplementation (10.30 g/kg) inhibited the induction of hepatic cytochrome P450 2E1 (CYP2E1), a major contributor to ethanol-mediated oxidative stress, and up-regulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream anti-oxidant protein heme oxygenase-1 (HO-1) in ethanol-treated mice.
These results indicate that the co-treatment with aqueous extract of Penthorum chinense (10.30 g/kg) protects against chronic ethanol-induced liver injury, possibly through suppressing CYP2E1-mediated oxidative stress and enhancing the oxidant defense systems via the activation of Nrf2/HO-1 pathway.
在中国，贯叶连翘（藤黄科）被用作苗族民族医学，用于治疗黄疸、胆囊炎、水肿、传染性肝炎和抗醉酒宿醉。本研究旨在探讨贯叶连翘对慢性乙醇诱导的肝损伤的可能保护作用。
小鼠用含酒精的 Lieber-DeCarli 液体饮食或含等热量麦芽糊精的对照饮食喂养，同时给予或不给予贯叶连翘水提物（PCP，5.15 和 10.30 g/kg/BW），共 4 周。水飞蓟素（86mg/kg）用作阳性对照，以比较 PCP 对慢性乙醇诱导肝毒性的疗效。
PCP（10.30g/kg）治疗可显著降低慢性乙醇暴露引起的血清 ALT 和 AST 水平升高、肝脂质堆积和炎症细胞因子（即 TNF-α、IL-6）。还发现 PCP 可减轻活性氧（ROS）生成和丙二醛（MDA）水平，恢复谷胱甘肽（GSH）耗竭，并增加超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性。此外，PCP 补充（10.30g/kg）可抑制肝细胞色素 P450 2E1（CYP2E1）的诱导，CYP2E1 是乙醇介导的氧化应激的主要贡献者，并上调核因子红细胞 2 相关因子 2（Nrf2）及其下游抗氧化蛋白血红素加氧酶-1（HO-1）在乙醇处理的小鼠中的表达。
这些结果表明，贯叶连翘水提物（10.30g/kg）的共同治疗可预防慢性乙醇诱导的肝损伤，可能通过抑制 CYP2E1 介导的氧化应激和通过激活 Nrf2/HO-1 途径增强抗氧化防御系统来实现。
