Gunduz Ergun, Arun Oguzhan, Bagci Sengal Taylan, Oc Bahar, Salman Alper, Yilmaz Setenay Arzu, Celik Cetin, Duman Ates
Department of Anesthesiology and Reanimation, Selcuk University, Faculty of Medicine, Konya, Turkey.
J Obstet Gynaecol Res. 2015 May;41(5):697-703. doi: 10.1111/jog.12624. Epub 2014 Dec 16.
To assess the effects of propofol and sevoflurane on the contraction elicited by dopamine, adrenaline and noradrenaline on isolated human umbilical arteries.
Umbilical arteries were cut into endothelium-denuded spiral strips and suspended in organ baths containing Krebs-Henseleit solution bubbled with O2 +CO2 mixture. Control contraction to phenylephrine (10(-5) M) was recorded. Response curves were obtained to 10(-5) M dopamine, 10(-5) M adrenaline or 10(-5) M noradrenaline. Afterwards, either cumulative propofol (10(-6) M, 10(-5) M and 10(-4) M) or cumulative sevoflurane (1.2%, 2.4% and 3.6%) was added to the organ bath, and the responses were recorded. Responses are expressed percentage of phenylephrine-induced contraction (mean ± standard deviation) (P < 0.05 = significance).
Propofol and sevoflurane elicited concentration-dependent relaxations in strips pre-contracted with dopamine, adrenaline and noradrenaline (P < 0.05). Highest (10(-4) M) concentration of propofol caused significantly higher relaxation compared with the highest (3.6%) concentration of sevoflurane in the contraction elicited by dopamine. High (10(-5) M) and highest concentrations of propofol caused significantly higher relaxation compared with the high (2.4%) and highest concentrations of sevoflurane on the contraction elicited by adrenaline. High and highest concentrations of sevoflurane caused significantly higher relaxation compared with the high and highest concentrations of propofol on the contraction elicited by noradrenaline.
Dopamine, adrenaline and noradrenaline elicit contractions in human umbilical arteries, and noradrenaline causes the highest contraction. Both propofol and sevoflurane inhibit these contractions in a dose-dependent manner. Propofol caused greater relaxation in the contractions elicited by dopamine and adrenaline while sevoflurane caused greater relaxation in the contraction elicited by noradrenaline.
评估丙泊酚和七氟醚对多巴胺、肾上腺素及去甲肾上腺素引起的人离体脐动脉收缩的影响。
将脐动脉剪成去内皮的螺旋条，悬挂于含 Krebs-Henseleit 溶液且通以 O₂+CO₂混合气体的器官浴槽中。记录对去氧肾上腺素（10⁻⁵ M）的对照收缩反应。获取对 10⁻⁵ M 多巴胺、10⁻⁵ M 肾上腺素或 10⁻⁵ M 去甲肾上腺素的反应曲线。之后，向器官浴槽中加入累积浓度的丙泊酚（10⁻⁶ M、10⁻⁵ M 和 10⁻⁴ M）或累积浓度的七氟醚（1.2%、2.4%和 3.6%），并记录反应。反应以去氧肾上腺素诱导的收缩的百分比表示（均值±标准差）（P < 0.05 为有统计学意义）。
丙泊酚和七氟醚使预先由多巴胺、肾上腺素及去甲肾上腺素收缩的条带产生浓度依赖性舒张（P < 0.05）。在多巴胺引起的收缩中，丙泊酚的最高浓度（10⁻⁴ M）比七氟醚的最高浓度（3.6%）引起的舒张显著更高。在肾上腺素引起的收缩中，丙泊酚的高浓度（10⁻⁵ M）和最高浓度比七氟醚的高浓度（2.4%）和最高浓度引起的舒张显著更高。在去甲肾上腺素引起的收缩中，七氟醚的高浓度和最高浓度比丙泊酚的高浓度和最高浓度引起的舒张显著更高。
多巴胺、肾上腺素及去甲肾上腺素可引起人脐动脉收缩，且去甲肾上腺素引起的收缩最强。丙泊酚和七氟醚均以剂量依赖性方式抑制这些收缩。丙泊酚在多巴胺和肾上腺素引起的收缩中产生更大的舒张，而七氟醚在去甲肾上腺素引起的收缩中产生更大的舒张。
