白细胞源性氧化剂对肌膜钠钾ATP酶及钙转运的影响。

Effects of leukocyte-derived oxidants on sarcolemmal Na,K,ATP-ase and calcium transport.

作者信息

Matsuoka T, Yanagishita T, Kako K J

机构信息

Department of Physiology, University of Ottawa, Ontario, Canada.

出版信息

Adv Exp Med Biol. 1989;248:621-8. doi: 10.1007/978-1-4684-5643-1_69.

DOI:10.1007/978-1-4684-5643-1_69

PMID:2551139

Abstract

Our study demonstrated that the Na,K,ATPase activity and ouabain binding sites were reduced by oxidants. Sarcolemmal calcium transport was also inhibited by hydrogen peroxide and HOC1. The action of HOC1 on the sarcolemmal functions was 2-3 orders of magnitude more powerful than that of hydrogen peroxide. Effects of hydrogen peroxide consisted of two components, i.e., the first, highly sensitive one, most probably mediated by Fe-catalyzed, site-specific free radical formation, and the second, less potent action by (high concentrations of) hydrogen peroxide. Finally, very low concentrations of hydrogen peroxide potentiated Na,K,ATPase activities when assayed using myocytes.

摘要

我们的研究表明，氧化剂可降低钠钾ATP酶活性和哇巴因结合位点。过氧化氢和次氯酸也会抑制肌膜钙转运。次氯酸对肌膜功能的作用比过氧化氢强2至3个数量级。过氧化氢的作用包括两个方面，即第一个是高度敏感的方面，很可能是由铁催化的位点特异性自由基形成介导的，第二个是（高浓度）过氧化氢的较弱作用。最后，当使用心肌细胞进行检测时，极低浓度的过氧化氢会增强钠钾ATP酶活性。