Squire K R, Embretson J E, First N L
Department of Meat and Animal Science, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
Am J Vet Res. 1989 Aug;50(8):1423-7.
The amphotropic murine leukemia virus (MLV) has been shown to infect mammalian species other than mice. If this virus infects and expresses genes in cells of livestock species (cattle, sheep, and pigs) it has potential for use as a vector to produce transgenic livestock. Because the gene-injection technique for producing transgenic animals is inherently inefficient, our laboratory was interested in identifying or constructing retroviral vectors capable of infecting livestock embryos. The infectivity of an amphotropic MLV-based vector for ovine, bovine, and porcine cells was tested. Experiments were also conducted to test the ability of the amphotropic MLV promoter, compared with known strong promoters, to express genes in cells from these species. Results indicated that amphotropic MLV infects and expresses genes efficiently in porcine cells and is, therefore, a potential vector for producing transgenic pigs. Infection was not detected in cells from adult bovine and ovine species; however, low levels of infection, with subsequent gene expression, were detected in cells derived from bovine embryos.
双嗜性鼠白血病病毒（MLV）已被证明可感染小鼠以外的哺乳动物物种。如果这种病毒在牲畜物种（牛、羊和猪）的细胞中感染并表达基因，那么它就有潜力用作生产转基因牲畜的载体。由于生产转基因动物的基因注射技术本质上效率低下，我们实验室对鉴定或构建能够感染牲畜胚胎的逆转录病毒载体很感兴趣。测试了一种基于双嗜性MLV的载体对绵羊、牛和猪细胞的感染性。还进行了实验，以测试双嗜性MLV启动子与已知强启动子相比，在这些物种的细胞中表达基因的能力。结果表明，双嗜性MLV在猪细胞中能高效感染并表达基因，因此是生产转基因猪的潜在载体。在成年牛和羊的细胞中未检测到感染；然而，在源自牛胚胎的细胞中检测到了低水平的感染以及随后的基因表达。
