Liu Jun, Chin-Sang Ian D
Department of Biology, Queen's University, 116 Barrie St., Kingston, ON K7L 3N6, Canada.
Methods. 2015 May;77-78:180-90. doi: 10.1016/j.ymeth.2014.12.009. Epub 2014 Dec 13.
PTEN (phosphatase and tensin homolog deleted on chromosome 10) has important roles in tumor suppression, metabolism, and development, yet its regulators, effectors, and functions are not fully understood. DAF-18 is the PTEN ortholog in Caenorhabditis elegans. DAF-18's role is highly conserved to human PTEN, and can be functionally replaced by human PTEN. Thus C. elegans provides a valuable model to study PTEN. This review assesses current and emerging methods to study DAF-18's regulators and functions in C. elegans. We propose genetic modify screens to identify genes that interact with daf-18/PTEN. These genes are potential targets for anticancer drug therapies. We also provide a review on the roles DAF-18/PTEN has during C. elegans development and how studying these physiological roles can provide mechanistic insight on DAF-18/PTEN function.
PTEN（第10号染色体缺失的磷酸酶及张力蛋白同源物）在肿瘤抑制、新陈代谢及发育过程中发挥着重要作用，但其调节因子、效应器及功能尚未完全明确。DAF-18是秀丽隐杆线虫中的PTEN直系同源基因。DAF-18的作用与人类PTEN高度保守，并且可以被人类PTEN在功能上替代。因此，秀丽隐杆线虫为研究PTEN提供了一个有价值的模型。本综述评估了当前及新兴的研究秀丽隐杆线虫中DAF-18调节因子及功能的方法。我们建议通过基因修饰筛选来鉴定与daf-18/PTEN相互作用的基因。这些基因是抗癌药物治疗的潜在靶点。我们还综述了DAF-18/PTEN在秀丽隐杆线虫发育过程中的作用，以及研究这些生理作用如何能够为DAF-18/PTEN的功能提供机制性见解。
