α-氯醛糖可打开青蛙离体感觉神经元的氯离子通道。

Alpha-chloralose opens the chloride channel of frog isolated sensory neurons.

作者信息

Ishizuka S, Sikdar S K, Yasui S, Oyama Y, Akaike N

机构信息

Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Brain Res. 1989 Sep 25;498(1):181-4. doi: 10.1016/0006-8993(89)90418-6.

DOI:10.1016/0006-8993(89)90418-6

PMID:2551455

Abstract

The effect of alpha-chloralose on the sensory neurons isolated enzymatically and mechanically from frog dorsal root ganglia was studied using a suction-pipette technique. The threshold concentration of alpha-chloralose was around 3 x 10(-5) M and the current produced by alpha-chloralose saturated at the concentration of 3 x 10(-3) M or more. The dose-response curve for alpha-chloralose provided a Ka value of 6 x 10(-4) M and a Hill coefficient of 1.8. The reversal potential of the response elicited by alpha-chloralose was close to the equilibrium potential for Cl- (ECl), indicating that the current was carried through Cl- channels. The current-voltage relationship indicated that there was little voltage dependence in the alpha-chloralose-induced response. The analysis of the variance of the alpha-chloralose-induced Cl- current fluctuations showed two types of the receptor-ionophore complexes with different channel conductances.

摘要

采用吸管技术研究了α-氯醛糖对从蛙背根神经节酶解和机械分离的感觉神经元的作用。α-氯醛糖的阈浓度约为3×10⁻⁵ M，α-氯醛糖产生的电流在3×10⁻³ M或更高浓度时达到饱和。α-氯醛糖的剂量反应曲线给出的Ka值为6×10⁻⁴ M，希尔系数为1.8。α-氯醛糖引发反应的反转电位接近Cl⁻的平衡电位（ECl），表明电流是通过Cl⁻通道传导的。电流-电压关系表明α-氯醛糖诱导的反应中电压依赖性很小。对α-氯醛糖诱导的Cl⁻电流波动的方差分析显示出两种具有不同通道电导的受体-离子载体复合物。