士的宁会降低海兔和青蛙神经节神经元的电压依赖性钙电流。
Strychnine decreases the voltage-dependent Ca2+ current of both Aplysia and frog ganglion neurons.
作者信息
Oyama Y, Akaike N, Carpenter D O
机构信息
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
出版信息
Cell Mol Neurobiol. 1988 Sep;8(3):307-14. doi: 10.1007/BF00711172.
Abstract
- The effects of strychnine on the voltage-dependent Ca2+ current (ICa) were studied in physically isolated Aplysia neurons and enzymatically dissociated frog sensory neurons of the dorsal root ganglion. Neurons were studied under the internal perfusion and the voltage clamp condition. 2. Strychnine decreased the ICa with threshold concentrations for effect at 1 to 10 microM. The depression of ICa increased with strychnine dose without effects on the current-voltage relation of ICa. The effects of low concentrations of strychnine were reversible, but recovery was incomplete at higher concentrations. The potency of strychnine was about 10 times less than that of diltiazem, an organic Ca2+ antagonist. At 100 microM the ICa of Aplysia neurons was reduced to about half of the control. This concentration of strychnine also reduced the peak amplitude of ICa of frog sensory neurons. 3. These results indicate that, in addition to its actions on transmitter responses and on Na+ and K+ currents, strychnine has effects on ICa that have not previously been appreciated.
摘要
- 在物理分离的海兔神经元和酶解的青蛙背根神经节感觉神经元中研究了士的宁对电压依赖性Ca2+电流（ICa）的影响。在内部灌注和电压钳制条件下对神经元进行研究。2. 士的宁降低ICa，产生效应的阈值浓度为1至10微摩尔。ICa的抑制作用随士的宁剂量增加而增强，且对ICa的电流-电压关系无影响。低浓度士的宁的作用是可逆的，但在较高浓度时恢复不完全。士的宁的效力约为有机Ca2+拮抗剂地尔硫䓬的十分之一。在100微摩尔时，海兔神经元的ICa降至对照的约一半。该浓度的士的宁也降低了青蛙感觉神经元ICa的峰值幅度。3. 这些结果表明，除了对递质反应以及对Na+和K+电流的作用外，士的宁对ICa也有影响，而这一点以前未被认识到。
相似文献
1
Strychnine decreases the voltage-dependent Ca2+ current of both Aplysia and frog ganglion neurons.士的宁会降低海兔和青蛙神经节神经元的电压依赖性钙电流。
Cell Mol Neurobiol. 1988 Sep;8(3):307-14. doi: 10.1007/BF00711172.
2
Some properties of inhibitory action of lidocaine on the Ca2+ current of single isolated frog sensory neurons.利多卡因对单个离体青蛙感觉神经元Ca2+电流抑制作用的一些特性。
Brain Res. 1988 Mar 1;442(2):223-8. doi: 10.1016/0006-8993(88)91507-7.
3
Inhibition of the voltage-dependent calcium currents in isolated frog sensory neurons by GABA-related agonistic compounds.
Neurosci Res. 1988 Dec;6(2):97-105. doi: 10.1016/0168-0102(88)90011-9.
4
Separation of ionic currents in the somatic membrane of frog sensory neurons.青蛙感觉神经元胞体膜中离子电流的分离
J Membr Biol. 1984;78(1):19-28. doi: 10.1007/BF01872528.
5
Characterization of an inward current elicited by edrophonium in physically isolated and internally perfused Aplysia neurons.在物理隔离且内部灌注的海兔神经元中对依酚氯铵引发的内向电流的特性描述。
Brain Res. 1988 Oct 25;463(1):124-32. doi: 10.1016/0006-8993(88)90534-3.
6
5-Hydroxytryptamine facilitates GABA-induced depolarization in bullfrog primary afferent neurons.5-羟色胺促进牛蛙初级传入神经元中γ-氨基丁酸诱导的去极化。
Neurosci Lett. 1988 Oct 17;92(3):270-4. doi: 10.1016/0304-3940(88)90601-5.
7
Mercury (Hg2+) decreases voltage-gated calcium channel currents in rat DRG and Aplysia neurons.汞（Hg2+）可降低大鼠背根神经节和海兔神经元中的电压门控钙通道电流。
Brain Res. 1993 Dec 31;632(1-2):121-6. doi: 10.1016/0006-8993(93)91146-j.
8
Ca2+-mediated suppression of the GABA-response through modulation of chloride channel gating in frog sensory neurones.
Neurosci Lett. 1988 Apr 12;86(3):311-6. doi: 10.1016/0304-3940(88)90502-2.
9
Alpha-chloralose opens the chloride channel of frog isolated sensory neurons.α-氯醛糖可打开青蛙离体感觉神经元的氯离子通道。
Brain Res. 1989 Sep 25;498(1):181-4. doi: 10.1016/0006-8993(89)90418-6.
10
A study of the cholinolytic actions of strychnine using the technique of concentration jump relaxation analysis.一项运用浓度跃变弛豫分析技术对士的宁胆碱能作用的研究。
Cell Mol Neurobiol. 1984 Sep;4(3):263-71. doi: 10.1007/BF00733589.
引用本文的文献
1
Glycinergic inhibition contributes to the generation of rostral scratch motor patterns in the turtle spinal cord.甘氨酸能抑制作用有助于海龟脊髓中吻侧抓挠运动模式的产生。
J Neurosci. 1997 May 1;17(9):3322-33. doi: 10.1523/JNEUROSCI.17-09-03322.1997.
2
Experimentally derived model for the locomotor pattern generator in the Xenopus embryo.非洲爪蟾胚胎中运动模式发生器的实验性衍生模型。
J Physiol. 1995 Dec 1;489 ( Pt 2)(Pt 2):489-510. doi: 10.1113/jphysiol.1995.sp021067.
3
Strychnine affects catecholamine secretion from bovine adrenal medulla chromaffin cells.士的宁影响牛肾上腺髓质嗜铬细胞中儿茶酚胺的分泌。
Naunyn Schmiedebergs Arch Pharmacol. 1995 Jul;352(1):11-6. doi: 10.1007/BF00169184.
4
Influences of trypsin and collagenase on acetylcholine responses of physically isolated single neurons of Aplysia californica.胰蛋白酶和胶原酶对加州海兔物理分离单个神经元乙酰胆碱反应的影响。
Cell Mol Neurobiol. 1990 Jun;10(2):193-205. doi: 10.1007/BF00734573.
5
Strychnine-induced potassium current in CA1 pyramidal neurones of the rat hippocampus.士的宁诱导的大鼠海马CA1锥体神经元钾电流
Br J Pharmacol. 1992 Aug;106(4):823-7. doi: 10.1111/j.1476-5381.1992.tb14419.x.
本文引用的文献
1
Effect of procaine on electrical properties of squid axon membrane.普鲁卡因对乌贼轴突膜电特性的影响。
Am J Physiol. 1959 May;196(5):1071-8. doi: 10.1152/ajplegacy.1959.196.5.1071.
2
The suction pipette method for internal perfusion and voltage clamp of small excitable cells.用于小的可兴奋细胞内部灌注和电压钳制的吸引移液管方法。
J Neurosci Methods. 1980 Feb;2(1):51-78. doi: 10.1016/0165-0270(80)90045-x.
3
Further analysis of inhibitory effects of propranolol and local anaesthetics on the calcium current in Helix neurones.普萘洛尔和局部麻醉药对河蜗神经元钙电流抑制作用的进一步分析。
Br J Pharmacol. 1982 May;76(1):37-43. doi: 10.1111/j.1476-5381.1982.tb09188.x.
4
Calcium currents in internally perfused nerve cell bodies of Limnea stagnalis.静水椎实螺内部灌注神经细胞体中的钙电流
J Physiol. 1982 Jan;322:503-28. doi: 10.1113/jphysiol.1982.sp014052.
5
Internal perfusion studies demonstrating GABA-induced chloride responses in frog primary afferent neurons.体内灌注研究表明，γ-氨基丁酸（GABA）可诱导青蛙初级传入神经元产生氯离子反应。
Am J Physiol. 1984 Mar;246(3 Pt 1):C259-65. doi: 10.1152/ajpcell.1984.246.3.C259.
6
A study of the cholinolytic actions of strychnine using the technique of concentration jump relaxation analysis.一项运用浓度跃变弛豫分析技术对士的宁胆碱能作用的研究。
Cell Mol Neurobiol. 1984 Sep;4(3):263-71. doi: 10.1007/BF00733589.
7
A low voltage-activated calcium conductance in embryonic chick sensory neurons.胚胎期鸡感觉神经元中的低电压激活钙电导。
Biophys J. 1984 Sep;46(3):413-8. doi: 10.1016/S0006-3495(84)84037-0.
8
Intracellular calcium ions and calcium currents in perfused neurones of the snail, Lymnaea stagnalis.椎实螺（Lymnaea stagnalis）灌流神经元中的细胞内钙离子和钙电流
J Physiol. 1984 Jul;352:637-52. doi: 10.1113/jphysiol.1984.sp015314.
9
Separation of ionic currents in the somatic membrane of frog sensory neurons.青蛙感觉神经元胞体膜中离子电流的分离
J Membr Biol. 1984;78(1):19-28. doi: 10.1007/BF01872528.
10
Receptor for ATP in the membrane of mammalian sensory neurones.哺乳动物感觉神经元膜上的ATP受体。
Neurosci Lett. 1983 Jan 31;35(1):41-5. doi: 10.1016/0304-3940(83)90524-4.
Zotero 插件