Wu Jinhua, Yang Zaiyue, Chen Ning, Zhu Wanying, Hong Junli, Huang Changgao, Zhou Xuemin
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China; Jiangsu Province Institute of Materia Media, Nanjing University of Technology, Nanjing 210029, PR China.
J Colloid Interface Sci. 2015 Mar 15;442:22-9. doi: 10.1016/j.jcis.2014.11.025. Epub 2014 Nov 18.
A molecularly imprinted stir bar was constructed based on Fe3O4@Polyaniline nanoparticles with magnetic field-induced self-assembly process. The monomer, methacrylic acid, was pre-assembled into the pre-polymers with vanillin as template by the formation of hydrogen bonds. After that, the magnetic complexes were generated by the hydrogen bonding, the hydrophobic and π-π interaction between the pre-polymers and Fe3O4@Polyaniline. The complexes were adsorbed on the surface of magnetic stir bar under the magnetic induction, and the coating of vanillin-molecularly imprinted polymers was generated by the one-step copolymerization basing on the cross linking of ethylene glycol dimethacrylate. The molecular imprinting stir bar showed superior selectivity and fast binding kinetics for vanillin, and was used for the enrichment of vanilla-flavor enhancers (vanillin, ethyl maltol and methyl vanillin) in infant milk powders. The results measured by HPLC-UV exhibited good linear ranges of 0.01-100, 0.02-100 and 0.03-100μgmL(-1) with the limit of detection of 2.5-10.0ngmL(-1), and the recoveries were 94.7-98.9%, 82.1-96.7% and 84.5-93.2% with RSD<7.2% for the three enhancers, respectively.
基于Fe3O4@聚苯胺纳米粒子，通过磁场诱导自组装过程构建了一种分子印迹搅拌棒。以香草醛为模板，通过氢键作用将单体甲基丙烯酸预组装成预聚物。之后，通过预聚物与Fe3O4@聚苯胺之间的氢键、疏水作用和π-π相互作用生成磁性复合物。在磁感应作用下，复合物吸附在磁性搅拌棒表面，并基于乙二醇二甲基丙烯酸酯的交联通过一步共聚生成香草醛分子印迹聚合物涂层。该分子印迹搅拌棒对香草醛表现出优异的选择性和快速结合动力学，并用于婴幼儿奶粉中香草味增强剂（香草醛、乙基麦芽酚和甲基香草醛）的富集。通过HPLC-UV测定的结果显示，线性范围良好，分别为0.01 - 100、0.02 - 100和0.03 - 100μgmL(-1)，检测限为2.5 - 10.0ngmL(-1)，三种增强剂的回收率分别为94.7 - 98.9%、82.1 - 96.7%和84.5 - 93.2%，相对标准偏差<7.2%。
