Sheedlo H J, Li L, Turner J E
Department of Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
Curr Eye Res. 1989 Jul;8(7):741-50. doi: 10.3109/02713688909025809.
Retinas of Royal College of Surgeons (RCS) dystrophic rats were investigated immunocytochemically for the distribution of the membrane-bound enzyme (Na+ + K+)-ATPase and the photo-pigment opsin prior to and during the retinal disease process. Retinas of 11 day-old dystrophic and control Long Evans rats showed (Na+ + K+)-ATPase immunostain most dense in the outer nuclear layer (ONL) and inner plexiform layer (IPL). Also, in these retinas, immunostaining for opsin was dense along rod inner segments (RIS) and on plasmalemma of ONL cell bodies and several cell bodies in the inner nuclear layer (INL). In retinas of control rats at 30 days and later, less dense (Na+ + K+)-ATPase immunostain was detected in the ONL than at 11 days and opsin-immunostained ROS were also detected. However, (Na+ + K+)-ATPase immunostained RIS were shorter in retinas of 30 day-old dystrophic than in retinas of age-matched control rats and an opsin-immunostained debris zone was also observed in dystrophic retinas. In retinas of 60 day-old dystrophic rats, the opsin-immunostained debris zone was more prominent than at 30 days, while the few ONL cell bodies immunostained for both proteins. Also, the outer IPL of 60 day-old dystrophic rat retinas immunostained more densely for (Na+ + K+)-ATPase than the inner IPL. In 120 day-old dystrophic rat retinas, (Na+ + K+)-ATPase immunostain was detected in the INL and IPL, while opsin staining was demonstrated only in the debris zone. This opsin-immunostained debris disappeared in a central-to-peripheral gradient. (Na+ + K+)-ATPase immunostain was still present in the INL and IPL in retinas and the optic nerve of one year-old RCS dystrophic rats; however, opsin was restricted to a few surviving cell bodies in the peripheral retina.
采用免疫细胞化学方法，对皇家外科学院（RCS）营养不良大鼠视网膜疾病发生前及发病过程中膜结合酶（Na⁺ + K⁺）-ATP酶和光色素视蛋白的分布情况进行了研究。11日龄营养不良和对照长 Evans 大鼠的视网膜显示，（Na⁺ + K⁺）-ATP酶免疫染色在外核层（ONL）和内网状层（IPL）最为密集。此外，在这些视网膜中，视蛋白免疫染色在杆状内节（RIS）以及 ONL 细胞体和内核层（INL）中的几个细胞体的质膜上较为密集。在30日龄及以后的对照大鼠视网膜中，ONL 中检测到的（Na⁺ + K⁺）-ATP酶免疫染色密度低于11日龄时，同时也检测到视蛋白免疫染色的视网膜外段（ROS）。然而，30日龄营养不良大鼠视网膜中（Na⁺ + K⁺）-ATP酶免疫染色的 RIS 比年龄匹配的对照大鼠视网膜中的短，并且在营养不良视网膜中还观察到一个视蛋白免疫染色的碎片区。在60日龄营养不良大鼠的视网膜中，视蛋白免疫染色的碎片区比30日龄时更为明显，而少数 ONL 细胞体对两种蛋白质均呈免疫染色。此外，60日龄营养不良大鼠视网膜的外 IPL 对（Na⁺ + K⁺）-ATP酶的免疫染色比内 IPL 更密集。在120日龄营养不良大鼠的视网膜中，在 INL 和 IPL 中检测到（Na⁺ + K⁺）-ATP酶免疫染色，而视蛋白染色仅在碎片区显示。这种视蛋白免疫染色的碎片以中央到周边的梯度消失。在1岁 RCS 营养不良大鼠的视网膜和视神经中，INL 和 IPL 中仍存在（Na⁺ + K⁺）-ATP酶免疫染色；然而，视蛋白仅限于周边视网膜中的少数存活细胞体。
