Zhang Xia, Liao Maoseng, Chang Dan, Zhang Fuchun
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Sheng li Road, Urumqi 830046, China.
BMC Res Notes. 2014 Dec 17;7:927. doi: 10.1186/1756-0500-7-927.
Much attention has been given to the potential of halophytes as sources of tolerance traits for introduction into cereals. However, a great deal remains unknown about the diverse mechanisms employed by halophytes to cope with salinity. To characterize salt tolerance mechanisms underlying Karelinia caspica, an Asteraceae halophyte, we performed Large-scale transcriptomic analysis using a high-throughput Illumina sequencing platform. Comparative gene expression analysis was performed to correlate the effects of salt stress and ABA regulation at the molecular level.
Total sequence reads generated by pyrosequencing were assembled into 287,185 non-redundant transcripts with an average length of 652 bp. Using the BLAST function in the Swiss-Prot, NCBI nr, GO, KEGG, and KOG databases, a total of 216,416 coding sequences associated with known proteins were annotated. Among these, 35,533 unigenes were classified into 69 gene ontology categories, and 18,378 unigenes were classified into 202 known pathways. Based on the fold changes observed when comparing the salt stress and control samples, 60,127 unigenes were differentially expressed, with 38,122 and 22,005 up- and down-regulated, respectively. Several of the differentially expressed genes are known to be involved in the signaling pathway of the plant hormone ABA, including ABA metabolism, transport, and sensing as well as the ABA signaling cascade.
Transcriptome profiling of K. caspica contribute to a comprehensive understanding of K. caspica at the molecular level. Moreover, the global survey of differentially expressed genes in this species under salt stress and analyses of the effects of salt stress and ABA regulation will contribute to the identification and characterization of genes and molecular mechanisms underlying salt stress responses in Asteraceae plants.
盐生植物作为引入谷物的耐受性性状来源的潜力已受到广泛关注。然而，关于盐生植物应对盐胁迫所采用的多种机制，仍有许多未知之处。为了表征菊科盐生植物里海补血草的耐盐机制，我们使用高通量Illumina测序平台进行了大规模转录组分析。进行了比较基因表达分析，以在分子水平上关联盐胁迫和脱落酸（ABA）调节的影响。
焦磷酸测序产生的总序列读数被组装成287,185个非冗余转录本，平均长度为652 bp。利用Swiss-Prot、NCBI nr、GO、KEGG和KOG数据库中的BLAST功能，共注释了216,416个与已知蛋白质相关的编码序列。其中，35,533个单基因被分类到69个基因本体类别中，18,378个单基因被分类到202条已知途径中。根据比较盐胁迫样本和对照样本时观察到的倍数变化，60,127个单基因差异表达，其中38,122个上调，22,005个下调。已知一些差异表达基因参与植物激素ABA的信号通路，包括ABA代谢、转运、感知以及ABA信号级联反应。
里海补血草的转录组分析有助于在分子水平上全面了解里海补血草。此外，对该物种在盐胁迫下差异表达基因的全面调查以及对盐胁迫和ABA调节影响的分析，将有助于鉴定和表征菊科植物盐胁迫反应的基因和分子机制。
