Jin Hangxia, Dong Dekun, Yang Qinghua, Zhu Danhua
Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People's Republic of China.
PLoS One. 2016 Mar 1;11(3):e0150504. doi: 10.1371/journal.pone.0150504. eCollection 2016.
Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes involved in salt tolerance and study halophilic mechanisms in this halophyte.
Illumina HiSeq 2500 was used to sequence cDNA libraries from salt-treated and control samples with three replicates each treatment. De novo assembly of the six transcriptomes identified 75,445 unigenes. A total of 23,901 (31.68%) unigenes were annotated. Compared with transcriptomes from the three salt-treated and three salt-free samples, 231 differentially expressed genes (DEGs) were detected (including 130 up-regulated genes and 101 down-regulated genes), and 195 unigenes were functionally annotated. Based on the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classifications of the DEGs, more attention should be paid to transcripts associated with signal transduction, transporters, the cell wall and growth, defense metabolism and transcription factors involved in salt tolerance.
This report provides a genome-wide transcriptional analysis of a halophyte, Suaeda glauca, under salt stress. Further studies of the genetic basis of salt tolerance in halophytes are warranted.
碱蓬是藜科多汁盐生植物，在中国沿海地区广泛分布。碱蓬对盐碱胁迫具有高度抗性。在本研究中，对碱蓬的盐响应转录组进行了分析，以鉴定参与耐盐性的基因，并研究这种盐生植物的嗜盐机制。
使用Illumina HiSeq 2500对盐处理和对照样品的cDNA文库进行测序，每个处理重复三次。对六个转录组进行从头组装，鉴定出75,445个单基因。共有23,901个（31.68%）单基因得到注释。与三个盐处理样品和三个无盐样品的转录组相比，检测到231个差异表达基因（DEG）（包括130个上调基因和101个下调基因），并且195个单基因得到了功能注释。根据基因本体论（GO）、直系同源基因簇（COG）和京都基因与基因组百科全书（KEGG）对DEG的分类，应更多关注与信号转导、转运蛋白、细胞壁和生长、防御代谢以及参与耐盐性的转录因子相关的转录本。
本报告提供了盐生植物碱蓬在盐胁迫下的全基因组转录分析。有必要进一步研究盐生植物耐盐性的遗传基础。
