Bao Jinli, Lin Lin
Department of Internal Medicine, Zaozhuang Fourth Municipal, Zaozhuang, 277100, Shandong Province, P.R.China,
J Thromb Thrombolysis. 2015 May;39(4):539-46. doi: 10.1007/s11239-014-1160-8.
Platelet apoptosis occurs commonly under various conditions such as physical and chemical stimuli. Acute coronary syndrome (ACS), one of most important cardiovascular and cerebrovascular diseases, severely threats people's health and is usually accompanied with various complications especially bleeding. There might be platelet apoptosis in ACS, which might be responsible for the complication of bleeding. The objective of the present study was to explore whether there were apoptotic platelets in ACS patients. Vein blood was drawn from eleven ACS patients and eleven health people. Platelet-rich plasma was prepared and subjected to apoptotic events analysis including increased expression of pro-apoptotic proteins and decreased expression of anti-apoptotic proteins, exposure of phosphatidylserine (PS), mitochondrial inner membrane potential depolarization and caspase-3 activation by Western blot and flow cytometry. In addition, washed platelets from the normal people were prepared and treated with the platelet-poor plasma (PPP) of the ACS patients, and were further examined apoptotic cascades. Paired Student's t test was used in the data comparisons. There were more platelets with depolarized mitochondrial inner membrane potential in the ACS patients than those of the health donors. Levels of Bax and Bak increased, while expression of Bcl-2 and Bcl-XL decreased in platelets from ACS patients. Caspase-3 activation was observed in platelets from the patients with ACS. Interestingly, there were significant differences in PS exposure between the platelets from the ACS patients and the normal controls. Furthermore, apoptotic events were observed in the normal platelets incubated with PPP from the ACS patients. In addition, pretreatment of healthy platelets with anti-oxidants N-acetyl cysteine (NAC) or dithiothreitol (DTT) significantly reduced ACS patients-derived PPP-induced platelet apoptosis. Platelets from the ACS patients are incurred apoptosis. Antioxidants NAC or DTT can reduce ACS patients-derived PPP-induced platelet apoptosis in vitro.
血小板凋亡在诸如物理和化学刺激等各种条件下普遍发生。急性冠状动脉综合征（ACS）是最重要的心血管疾病之一，严重威胁人们的健康，通常伴有各种并发症，尤其是出血。ACS患者可能存在血小板凋亡，这可能是出血并发症的原因。本研究的目的是探讨ACS患者中是否存在凋亡血小板。从11例ACS患者和11名健康人抽取静脉血。制备富含血小板的血浆并进行凋亡事件分析，包括促凋亡蛋白表达增加和抗凋亡蛋白表达减少、磷脂酰丝氨酸（PS）暴露、线粒体内膜电位去极化以及通过蛋白质印迹法和流式细胞术检测半胱天冬酶-3激活。此外，制备正常人的洗涤血小板并用ACS患者的乏血小板血浆（PPP）处理，并进一步检测凋亡级联反应。数据比较采用配对学生t检验。ACS患者中线粒体内膜电位去极化的血小板比健康供体的更多。ACS患者血小板中Bax和Bak水平升高，而Bcl-2和Bcl-XL表达降低。在ACS患者的血小板中观察到半胱天冬酶-3激活。有趣的是，ACS患者的血小板与正常对照之间在PS暴露方面存在显著差异。此外，在用ACS患者的PPP孵育的正常血小板中观察到凋亡事件。此外，用抗氧化剂N-乙酰半胱氨酸（NAC）或二硫苏糖醇（DTT）预处理健康血小板可显著减少ACS患者来源的PPP诱导的血小板凋亡。ACS患者的血小板发生凋亡。抗氧化剂NAC或DTT可在体外减少ACS患者来源的PPP诱导的血小板凋亡。
