Rosewell Katherine L, Al-Alem Linah, Zakerkish Farnosh, McCord Lauren, Akin James W, Chaffin Charles L, Brännström Mats, Curry Thomas E
Department of Obstetrics and Gynecology, University of Kentucky College of Medicine, Lexington, Kentucky.
Gynaecology and Reproductive Medicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Fertil Steril. 2015 Mar;103(3):826-33. doi: 10.1016/j.fertnstert.2014.11.017. Epub 2014 Dec 13.
To explore the temporal expression in granulosa and theca cells of key members of the MMP and ADAMTS families across the periovulatory period in women to gain insight into their possible roles during ovulation and early luteinization.
Experimental prospective clinical study and laboratory-based investigation.
University medical center and private IVF center.
ANIMAL AND PATIENT(S): Thirty-eight premenopausal women undergoing surgery for tubal ligation and six premenopausal women undergoing assisted reproductive techniques.
INTERVENTION(S): Administration of hCG and harvesting of follicles by laparoscopy and collection of granulosa-lutein cells at oocyte retrieval.
MAIN OUTCOME MEASURE(S): Expression of mRNA for matrix metalloproteinase (MMPs) and the A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS) in human granulosa cells and theca cells collected across the periovulatory period of the menstrual cycle and in cultured granulosa-lutein cells after hCG. Localization of MMPs and ADAMTSs by immunohistochemistry.
RESULT(S): Expression of MMP1 and MMP19 mRNA increased in both granulosa and theca cells after hCG administration. ADAMTS1 and ADAMTS9 mRNA increased in granulosa cells after hCG treatment, however, thecal cell expression for ADAMTS1 was unchanged, while ADAMTS9 expression was decreased. Expression of MMP8 and MMP13 mRNA was unchanged. Immunohistochemistry confirmed the localization of MMP1, MMP19, ADAMTS1, and ADAMTS9 to the granulosa and thecal cell layers.
CONCLUSION(S): The collection of the dominant follicle throughout the periovulatory period has allowed the identification of proteolytic remodeling enzymes in the granulosa and theca compartments that may be critically involved in human ovulation. These proteinases may work in concert to regulate breakdown of the follicular wall and release of the oocyte.
探讨女性围排卵期颗粒细胞和卵泡膜细胞中基质金属蛋白酶（MMP）家族和含血小板反应蛋白基序的解聚素样金属蛋白酶（ADAMTS）家族关键成员的时间表达情况，以深入了解它们在排卵和早期黄体化过程中的可能作用。
实验性前瞻性临床研究及基于实验室的调查。
大学医学中心和私立体外受精中心。
38名接受输卵管结扎手术的绝经前女性和6名接受辅助生殖技术的绝经前女性。
给予人绒毛膜促性腺激素（hCG），通过腹腔镜检查采集卵泡，并在取卵时收集颗粒黄体细胞。
月经周期围排卵期采集的人颗粒细胞和卵泡膜细胞以及hCG处理后的培养颗粒黄体细胞中基质金属蛋白酶（MMPs）和含血小板反应蛋白基序的解聚素样金属蛋白酶（ADAMTS）的mRNA表达。通过免疫组织化学法对MMPs和ADAMTSs进行定位。
给予hCG后，颗粒细胞和卵泡膜细胞中MMP1和MMP19 mRNA的表达均增加。hCG处理后，颗粒细胞中ADAMTS1和ADAMTS9 mRNA增加，然而，卵泡膜细胞中ADAMTS1的表达未改变，而ADAMTS9的表达降低。MMP8和MMP13 mRNA的表达未改变。免疫组织化学证实MMP1、MMP19、ADAMTS1和ADAMTS9定位于颗粒细胞层和卵泡膜细胞层。
在围排卵期采集优势卵泡，使得在颗粒细胞和卵泡膜细胞区室中鉴定出可能在人类排卵过程中起关键作用的蛋白水解重塑酶成为可能。这些蛋白酶可能协同作用，调节卵泡壁的分解和卵母细胞的释放。
