与高强度聚焦超声消融引起的温度升高相关的电生理变化。
Electrophysiological changes correlated with temperature increases induced by high-intensity focused ultrasound ablation.
作者信息
Wu Ziqi, Kumon Ronald E, Laughner Jacob I, Efimov Igor R, Deng Cheri X
机构信息
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Department of Biomedical Engineering, Washington University at Saint Louis, MO, USA.
出版信息
Ultrasound Med Biol. 2015 Feb;41(2):432-48. doi: 10.1016/j.ultrasmedbio.2014.09.009. Epub 2014 Dec 13.
To gain better understanding of the detailed mechanisms of high-intensity focused ultrasound (HIFU) ablation for cardiac arrhythmias, we investigated how the cellular electrophysiological (EP) changes were correlated with temperature increases and thermal dose (cumulative equivalent minutes [CEM43]) during HIFU application using Langendorff-perfused rabbit hearts. Employing voltage-sensitive dye di-4-ANEPPS, we measured the EP and temperature during HIFU using simultaneous optical mapping and infrared imaging. Both action potential amplitude (APA) and action potential duration at 50% repolarization (APD50) decreased with temperature increases, and APD50 was more thermally sensitive than APA. EP and tissue changes were irreversible when HIFU-induced temperature increased above 52.3 ± 1.4°C and log10(CEM43) above 2.16 ± 0.51 (n = 5), but were reversible when temperature was below 50.1 ± 0.8°C and log10(CEM43) below -0.9 ± 0.3 (n = 9). EP and temperature/thermal dose changes were spatially correlated with HIFU-induced tissue necrosis surrounded by a transition zone.
为了更好地理解高强度聚焦超声（HIFU）消融治疗心律失常的详细机制，我们使用Langendorff灌注兔心研究了在HIFU应用过程中细胞电生理（EP）变化与温度升高和热剂量（累积等效分钟数 [CEM43]）之间的相关性。我们使用电压敏感染料di-4-ANEPPS，通过同步光学映射和红外成像测量了HIFU应用过程中的EP和温度。随着温度升高，动作电位幅度（APA）和复极化50%时的动作电位时程（APD50）均降低，且APD50比APA对热更敏感。当HIFU诱导的温度升高超过52.3±1.4°C且log10（CEM43）超过2.16±0.51（n = 5）时，EP和组织变化是不可逆的，但当温度低于50.1±0.8°C且log10（CEM43）低于 -0.9±0.3（n = 9）时，变化是可逆的。EP和温度/热剂量变化在空间上与HIFU诱导的组织坏死及其周围的过渡区相关。
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