肝片吸虫和巨片吸虫肝脏中某些重金属的生物累积
Bioaccumulation of Some Heavy Metals in the Liver Flukes Fasciola hepatica and F. gigantica.
作者信息
Lotfy Wael Mohamed, Ezz Anwar Mohsen, Hassan Ashraf Ali Moustafa
机构信息
Parasitology Department, Medical Research Institute, Alexandria University, Egypt.
Applied Medical Chemistry Department, Medical Research institute, Alexandria University, Egypt.
出版信息
Iran J Parasitol. 2013 Oct;8(4):552-8.
BACKGROUND
Heavy metals tend to bioaccumulate in living organisms, and their accumulation has been a major concern. As mammals are known to excrete heavy metals via their bile, it seems to be very promising to analyse metal burdens of parasites that infect the biliary tree such as liver flukes of the genus Fasciola. The present study was carried out to evaluate F. hepatica and F. gigantica as bioaccumulators of heavy metals, and to estimate their use as sensitive markers of environmental pollution with heavy metals.
METHODS
A total of 36 slaughtered buffaloes (26 infected and 10 controls) collected from the slaughter-house of Tanta City, Egypt were used. Samples of muscle and liver tissues were taken from each buffalo. A total of 44 adult Fasciola flukes were collected from the 26 infected buffaloes. Quantification of some heavy metals (Cd, Cr, Cu, Pb and Zn) in samples was carried out using electrothermal atomic absorption spectrometry.
RESULTS
Results revealed different concentrations of heavy metals in different host tissues. The adult flukes were classified into F. hepatica (n = 25) and F. gigantica (n = 19). The bio-concentration factor (BCF) of Cr was significantly higher in F. hepatica (P = 0.0465) while BCF of Zn was significantly higher in F. gigantica (P = 0.0189). A comparative study between the two species as regards the BCF was never done before.
CONCLUSION
The obtained results indicate the possibility of use of Fasciola flukes as markers of environmental pollution with some heavy metals.
背景
重金属往往会在生物体内生物累积，其累积一直是人们主要关注的问题。由于已知哺乳动物通过胆汁排泄重金属，因此分析感染胆管的寄生虫（如片形吸虫属的肝吸虫）的金属负荷似乎非常有前景。本研究旨在评估肝片吸虫和巨片吸虫作为重金属生物累积者的情况，并估计它们作为重金属环境污染敏感标志物的用途。
方法
使用了从埃及坦塔市屠宰场收集的36头屠宰水牛（26头感染，10头对照）。从每头水牛身上采集肌肉和肝脏组织样本。从26头感染的水牛身上总共收集了44条成年肝吸虫。使用电热原子吸收光谱法对样本中的一些重金属（镉、铬、铜、铅和锌）进行定量分析。
结果
结果显示不同宿主组织中重金属浓度不同。成年吸虫分为肝片吸虫（n = 25）和巨片吸虫（n = 19）。肝片吸虫中铬的生物浓缩系数（BCF）显著更高（P = 0.0465），而巨片吸虫中锌的BCF显著更高（P = 0.0189）。此前从未对这两个物种的BCF进行过比较研究。
结论
所得结果表明肝吸虫有可能作为某些重金属环境污染的标志物。
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