降钙素基因相关肽对大鼠主动脉平滑肌的作用

Action of calcitonin gene-related peptide on rat aortic smooth muscle.

作者信息

Vallotton M B, Dolci W, Gerber-Wicht C, Fischer J A

机构信息

Department of Medicine, University of Geneva, Switzerland.

出版信息

Eur J Pharmacol. 1989 Jul 18;166(2):219-22. doi: 10.1016/0014-2999(89)90062-9.

DOI:10.1016/0014-2999(89)90062-9

PMID:2551700

Abstract

The effects of rat calcitonin gene-related peptide (CGRP) on superfused rat aortic smooth muscle cells in cell culture were investigated. Exposure of the cells for 10 min to CGRP (10(-7) M), with or without pretreatment with pertussis toxin, stimulated the release of cyclic AMP but not of prostacyclin, as judged by radioimmunoassay of its stable metabolite, 6-keto-PGF1 alpha. Pretreatment of the cells with pertussis toxin did not alter the response to CGRP. The direct action of CGRP on smooth muscle cells and on the CGRP-induced formation of cyclic AMP did not appear to depend on the production of prostacyclin in these vascular smooth muscle cells.

摘要

研究了大鼠降钙素基因相关肽（CGRP）对细胞培养中经超灌流的大鼠主动脉平滑肌细胞的影响。通过对其稳定代谢产物6-酮-前列环素F1α进行放射免疫测定判断，将细胞暴露于CGRP（10⁻⁷M）10分钟，无论是否用百日咳毒素预处理，均可刺激环磷酸腺苷（cAMP）释放，但不刺激前列环素释放。用百日咳毒素预处理细胞并未改变其对CGRP的反应。CGRP对平滑肌细胞的直接作用以及CGRP诱导的环磷酸腺苷形成似乎并不依赖于这些血管平滑肌细胞中前列环素的产生。