降钙素基因相关肽刺激大鼠主动脉平滑肌细胞中环磷酸腺苷的形成。

Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells.

作者信息

Kubota M, Moseley J M, Butera L, Dusting G J, MacDonald P S, Martin T J

出版信息

Biochem Biophys Res Commun. 1985 Oct 15;132(1):88-94. doi: 10.1016/0006-291x(85)90992-1.

Abstract

In rat aortic smooth muscle cells in culture, calcitonin gene-related peptide stimulated cAMP formation in a dose-dependent manner, half-maximally effective at 0.5 to 1 nM. There was no effect on formation of cGMP, which was increased 300-fold in the same experiments by atriopeptin or sodium nitroprusside. The vasodilator effect of CGRP in rat aorta requires an intact endothelium, indicating that increase in vascular smooth muscle cAMP is not in itself sufficient to bring about relaxation. cAMP is probably a mediator of CGRP action in vascular smooth muscle.

摘要

在培养的大鼠主动脉平滑肌细胞中，降钙素基因相关肽以剂量依赖方式刺激环磷酸腺苷（cAMP）的生成，在0.5至1纳摩尔时达到最大效应的一半。对环磷酸鸟苷（cGMP）的生成没有影响，而在相同实验中，心房肽或硝普钠可使cGMP增加300倍。降钙素基因相关肽在大鼠主动脉中的血管舒张作用需要完整的内皮，这表明血管平滑肌中cAMP的增加本身不足以引起舒张。cAMP可能是降钙素基因相关肽在血管平滑肌中发挥作用的介质。