Rasmussen U B, Köhrle J, Rokos H, Hesch R D
Abteilung Endokrinologie, Medizinische Hochschule Hannover, FRG.
FEBS Lett. 1989 Sep 25;255(2):385-90. doi: 10.1016/0014-5793(89)81128-7.
N-bromoacetyl-3,3',5-tri[3'-125I]iodo-L-thyronine was used to label intact heart mitochondria from eu, hypo- and hyperthyroid rats in order to identify proteins involved in T3-regulated mitochondrial processes. The results show strong labeling, competed for by T3 and other analogues, of two proteins with a molecular mass of 48,000 and 49,200 Da. No labeling is seen of the adenine nucleotide translocase, a likely target, neither at 0 degree C, at room temperature, nor after preincubation with the substrates or specific inhibitors. No difference in labeling intensity or distribution is seen in mitochondria from eu-, hypo- or hyperthyroid rats, and the abundance of the adenine nucleotide translocase is unchanged, but five other proteins show differential abundance.
使用N-溴乙酰基-3,3',5-三[3'-¹²⁵I]碘-L-甲状腺素对正常甲状腺、甲状腺功能减退和甲状腺功能亢进大鼠的完整心脏线粒体进行标记，以鉴定参与T3调节的线粒体过程的蛋白质。结果显示，两种分子量分别为48,000和49,200 Da的蛋白质有强烈的标记，T3和其他类似物可与之竞争。在0℃、室温下，以及与底物或特异性抑制剂预孵育后，均未观察到可能的靶点——腺嘌呤核苷酸转位酶有标记。正常甲状腺、甲状腺功能减退或甲状腺功能亢进大鼠的线粒体在标记强度或分布上没有差异，腺嘌呤核苷酸转位酶的丰度没有变化，但其他五种蛋白质显示出丰度差异。
