Johansen T, Holm T, Bjørklid E
Institute of Medical Biology, University of Tromsø, Norway.
Gene. 1989 Jul 15;79(2):259-67. doi: 10.1016/0378-1119(89)90208-4.
A cDNA clone homologous to the RTVL-H family of human retrovirus-like elements was isolated from a human placenta cDNA library. The nucleotide sequence of the 1084-bp cDNA revealed an open reading frame (ORF) that may encode a 146 amino acid protein with significant homology to retroviral proteases. Downstream from the putative protease ORF a 3' long terminal repeat (LTR) containing U3 and R regions was found. The cDNA sequence ends in a poly(A) tail appropriately positioned downstream from a polyadenylation signal in the LTR. Northern-blot analysis showed that several distinct RTVL-H homologous transcripts are present in human placenta. We also show that repetitive RTVL-H homologous sequences are present in the genomes of both gorilla and African green monkey.
从人胎盘cDNA文库中分离出一个与人逆转录病毒样元件RTVL-H家族同源的cDNA克隆。1084个碱基对的cDNA的核苷酸序列显示出一个开放阅读框（ORF），它可能编码一种与逆转录病毒蛋白酶具有显著同源性的146个氨基酸的蛋白质。在假定的蛋白酶开放阅读框下游发现了一个包含U3和R区域的3'长末端重复序列（LTR）。cDNA序列在LTR中聚腺苷酸化信号下游适当位置的聚（A）尾处结束。Northern印迹分析表明，人胎盘中存在几种不同的RTVL-H同源转录本。我们还表明，大猩猩和非洲绿猴的基因组中都存在重复的RTVL-H同源序列。
