布氏锥虫的微管蛋白基因簇起始于一个完整的β基因，终止于一个被类似逆转录转座子序列打断的截短β基因。

The tubulin gene cluster of Trypanosoma brucei starts with an intact beta-gene and ends with a truncated beta-gene interrupted by a retrotransposon-like sequence.

作者信息

Affolter M, Rindisbacher L, Braun R

机构信息

Institut für Allgemeine Mikrobiologie, Universität Bern, Switzerland.

出版信息

Gene. 1989 Aug 1;80(1):177-83. doi: 10.1016/0378-1119(89)90264-3.

DOI:10.1016/0378-1119(89)90264-3

PMID:2551783

Abstract

In Trypanosoma brucei the genes coding for alpha- and beta-tubulin are arranged in a single cluster comprising about ten alternating alpha and beta genes. The cluster starts at its 5' end with an intact beta gene. In this area, a 1.1-kb segment upstream of the mini-exon addition site has been sequenced. Several potential transcription signals were seen. The 3' end of the cluster terminates in the middle of a beta gene, making it an inactive truncated pseudogene. The adjoining 1.7-kb sequence shows a high degree of similarity with a retrotransposon-like (RTnL) element previously characterized elsewhere in the trypanosome genome. This is the first report of an RTnL element interrupting a protein-coding gene of T. brucei.

摘要

在布氏锥虫中，编码α-微管蛋白和β-微管蛋白的基因排列在一个单一的簇中，该簇包含大约十个交替的α和β基因。该簇在其5'端以一个完整的β基因开始。在这个区域，已对小外显子添加位点上游1.1 kb的片段进行了测序。发现了几个潜在的转录信号。该簇的3'端在一个β基因的中间终止，使其成为一个无活性的截短假基因。相邻的1.7 kb序列与先前在锥虫基因组其他地方鉴定的类反转录转座子（RTnL）元件具有高度相似性。这是关于RTnL元件中断布氏锥虫蛋白质编码基因的首次报道。