Ouban Abderrahman, Ahmed Atif
*Department of Pathology, College of Medicine, Alfaisal University †Department of Pathology, King Fahd Medical City, Riyadh, Kingdom of Saudi Arabia.
Appl Immunohistochem Mol Morphol. 2015 Jul;23(6):444-8. doi: 10.1097/PAI.0000000000000104.
Claudins are the main sealing proteins of the intercellular tight junctions and play an important role in cancer cell progression and dissemination. The authors have previously shown that overexpression of claudin-1 is associated with angiolymphatic and perineural invasion, consistent with aggressive tumor behavior and with advanced stage disease in oral squamous cell carcinomas (OSCCs). Our goal in this study was to examine claudin-1 expression in a tissue microarray of OSCCs taken from multiple sites within the oral cavity.
This study examined and compared the expression of claudin-1 by immunohistochemistry in 60 tissue samples (49 OSCCs and 10 cases of non-neoplastic tissue, single core per case) were analyzed for claudin-1 expression by immunohistochemistry. The tumors included SCCs from the tongue (n=28), the cheek (n=9), gingival (n=4), lip (n=3), and oral cavity (n=5). Nonmalignant normal oral mucosa from the tongue (unmatched cases, n=2). Cancer adjacent tissue samples were taken from the tongue (n=6), gingival (n=2), and palate (n=1).
This study demonstrates the expression of claudin-1 protein across a sample of OSCCs originating from multiple locations in the oral cavity. The highest expression of claudin-1 was observed in well-differentiated OSCCs, whereas poorly differentiated OSCCs exhibited mostly negative staining for claudin-1. In addition, we hereby report differential pattern of expression among tumors of different sites within the oral cavity, and between benign and cancerous samples. Our understanding of the exact function and role of claudin-1 in tumorigenesis is expanding exponentially.
紧密连接蛋白是细胞间紧密连接的主要密封蛋白，在癌细胞进展和扩散中起重要作用。作者之前已经表明，紧密连接蛋白-1的过表达与血管淋巴管浸润和神经周围浸润相关，这与口腔鳞状细胞癌（OSCC）的侵袭性肿瘤行为和晚期疾病一致。我们在本研究中的目标是检测取自口腔多个部位的OSCC组织芯片中紧密连接蛋白-1的表达。
本研究通过免疫组织化学检测并比较了60个组织样本（49例OSCC和10例非肿瘤组织，每例单核）中紧密连接蛋白-1的表达。肿瘤包括来自舌（n = 28）、颊（n = 9）、牙龈（n = 4）、唇（n = 3）和口腔（n = 5）的鳞状细胞癌。来自舌的非恶性正常口腔黏膜（不匹配病例，n = 2）。癌旁组织样本取自舌（n = 6）、牙龈（n = 2）和腭（n = 1）。
本研究证明了紧密连接蛋白-1蛋白在源自口腔多个部位的OSCC样本中的表达。在高分化OSCC中观察到紧密连接蛋白-1的最高表达，而低分化OSCC对紧密连接蛋白-1大多呈阴性染色。此外，我们在此报告了口腔内不同部位肿瘤之间以及良性和癌性样本之间的差异表达模式。我们对紧密连接蛋白-1在肿瘤发生中的确切功能和作用的理解正在呈指数级扩展。
