用于分离大鼠肾上腺细胞类固醇生成的柱灌注系统。
Column perifusion system for steroidogenesis in isolated rat adrenal cells.
作者信息
Matsuki M, Ikeda M, Watanabe S, Nishida S, Horino M, Saito T
机构信息
Department of Medicine, Kawasaki Medical School, Okayama, Japan.
出版信息
Horm Metab Res. 1989 Aug;21(8):431-3. doi: 10.1055/s-2007-1009254.
A perifusion system using a plastic column into which isolated rat adrenal cells had been installed was attempted. After ACTH or cAMP was administered to the column, the corticosterone concentration in the eluate was determined. ACTH in 10(-13) and 10(-12) M did not promote corticosterone production, whereas 10(-11) and 10(-10) M showed a dose dependent production of corticosterone. By iterative infusion of 10(-11) or 10(-9) M of ACTH, very clear responses to restimulation of ACTH were noted. Following the administrations of 10(-3) or 10(-2) M of dibutyryl adenosine 3',5'-cyclic monophosphate (dbcAMP), the production of corticosterone increased dose-dependently. These results suggest that this perifusion system is effective for examining the effects of ACTH or cAMP on steroidogenesis of cells.
尝试构建一种灌流系统，该系统使用一个装有分离的大鼠肾上腺细胞的塑料柱。向该柱中加入促肾上腺皮质激素（ACTH）或环磷酸腺苷（cAMP）后，测定洗脱液中皮质酮的浓度。10(-13) M和10(-12) M的ACTH不能促进皮质酮的产生，而10(-11) M和10(-10) M的ACTH则呈现出剂量依赖性的皮质酮产生。通过反复注入10(-11) M或10(-9) M的ACTH，可观察到对ACTH再刺激的非常明显的反应。给予10(-3) M或10(-2) M的二丁酰腺苷3',5'-环一磷酸（dbcAMP）后，皮质酮的产生呈剂量依赖性增加。这些结果表明，该灌流系统对于检测ACTH或cAMP对细胞类固醇生成的影响是有效的。
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