Ma Xiao-Li, Zou Ping-Ping, Lei Wei, Tu Peng-Fei, Jiang Yong
Yao Xue Xue Bao. 2014 Sep;49(9):1248-57.
Quantitative NMR (qNMR) is a technology based on the principle of NMR. This technology does not need the references of the determined components, which supplies a solution for the problem of reference scarcity in the quantitative analysis of traditional Chinese medicines. Moreover, this technology has the advantages of easy operation, non-destructiveness for the determined sample, high accuracy and repeatability, in comparison with HPLC, LC-MS and GC-MS. NMR technology has achieved quantum leap in sensitivity and accuracy with the development of NMR hardware. In addition, the choice of appropriate experimental parameters of the pre-treatment and measurement procedure as well as the post-acquisition processing is also important for obtaining high-quality and reproducible NMR spectra. This review summarizes the principle of qHNMR, the various experimental parameters affecting the accuracy and the precision of qHNMR, such as signal to noise ratio, relaxation delay, pulse width, acquisition time, window function, phase correction and baseline correction, and their corresponding optimized methods. Moreover, the application of qHNMR in the fields of quantitation of single or multi-components of traditional Chinese medicines, the purity detection of references, and the quality analysis of foods has been discussed. In addition, the existing questions and the future application prospects of qNMR in natural product areas are also presented.
定量核磁共振（qNMR）是一种基于核磁共振原理的技术。该技术无需被测成分的对照品，为解决中药定量分析中对照品匮乏的问题提供了一种解决方案。此外，与高效液相色谱（HPLC）、液相色谱 - 质谱联用（LC-MS）和气相色谱 - 质谱联用（GC-MS）相比，该技术具有操作简便、对被测样品无损、准确度高和重复性好等优点。随着核磁共振硬件的发展，核磁共振技术在灵敏度和准确度方面实现了飞跃。此外，选择合适的预处理、测量程序的实验参数以及采集后处理对于获得高质量和可重复的核磁共振谱图也很重要。本综述总结了定量氢核磁共振（qHNMR）的原理、影响qHNMR准确度和精密度的各种实验参数，如信噪比、弛豫延迟、脉冲宽度、采集时间、窗函数、相位校正和基线校正及其相应的优化方法。此外，还讨论了qHNMR在中药单一组分或多组分定量、对照品纯度检测以及食品质量分析领域的应用。此外，还介绍了qNMR在天然产物领域存在的问题和未来的应用前景。
