Adwan Ghaleb, Adwan Kamel, Jarrar Naser, Amleh Alaa
Department of Biology and Biotechnology, an-Najah National University, Nablus, Palestine
Roum Arch Microbiol Immunol. 2014 Jan-Jun;73(1-2):9-17.
This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamides, streptogramin, aminoglycoside and tetracycline resistance genes among MRSA isolates. A total of 55 clinical MRSA isolates were included in this study, antibiotic resistance was conducted by Kirby-Bauer disk diffusion method, broth microdilution assay and multiplex PCR technique. Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay. The prevalence of erm(A), erm(C), tetK, tetM, aacA-aphD, vat(A), vat(B) and vat(C) gene among MRSA isolates was 30.9%, 74.5%, 76.4%, 16.4%, 74.5%, 1.8%, 0% and 5.5%, respectively. These MRSA strains belonged to SCCmec types II, III, IVa and V. Rapid and reliable method for antibiotic susceptibility is important to determine the appropriate therapy decision. Multiplex PCR can be used for confirmation of the results obtained by disk diffusion method or could be used as an alternative diagnostic method in the routine diagnosis for rapid, sensitive, and specific detection of MRSA associated antibiotic resistance genes.
本研究旨在评估表型抗生素敏感性模式与抗生素耐药基因之间的关系，并调查耐甲氧西林金黄色葡萄球菌（MRSA）分离株中大环内酯类、林可酰胺类、链阳菌素类、氨基糖苷类和四环素类耐药基因的流行情况。本研究共纳入55株临床MRSA分离株，采用 Kirby-Bauer 纸片扩散法、肉汤微量稀释法和多重PCR技术进行抗生素耐药性检测。我们的结果表明，传统药敏试验与多重PCR法基因检测之间不存在不一致性。MRSA分离株中erm(A)、erm(C)、tetK、tetM、aacA-aphD、vat(A)、vat(B)和vat(C)基因的流行率分别为30.9%、74.5%、76.4%、16.4%、74.5%、1.8%、0%和5.5%。这些MRSA菌株属于SCCmec II型、III型、IVa型和V型。快速可靠的抗生素敏感性检测方法对于确定合适的治疗方案至关重要。多重PCR可用于确认纸片扩散法获得的结果，或可作为常规诊断中快速、灵敏、特异检测MRSA相关抗生素耐药基因的替代诊断方法。
