Kaigorodova Evgeniya V, Zavyalova Marina V, Bogatyuk Maria V, Tarabanovskaya Natalia A, Slonimskaya Elena M, Perelmuter Vladimir M
Tomsk Cancer Research Institute, Tomsk, Russian Federation Laboratory for Translational Cellular and Molecular Biomedicine, Tomsk State University, Tomsk, Russian Federation Siberian State Medical University, Tomsk, Russian Federation.
Tomsk Cancer Research Institute, Tomsk, Russian Federation.
Cancer Biomark. 2015;15(2):143-50. doi: 10.3233/CBM-140446.
Heat shock protein beta-1 (HspB1) is a chaperone of the sHsp (small heat shock protein). The common functions of sHsps are chaperone activity, inhibition of apoptosis, regulation of cell development, and cell differentiation, take part in signal transduction.
To study the intracellular localization of phosphorylated features and non-phosphorylated forms of HspB1 in primary breast cancer cells and to evaluate their relationship with regional lymphatic metastasis.
Tumor biopsies of breast tissue were collected from 100 patients with a confirmed diagnosis of invasive carcinoma, nonspecific type, between the ages of 31-80 years. Immunohistochemistry was used to determine the intracellular localization of phosphorylated and non-phosphorylated forms of HspB1.
The result of this study showed that biopsies from patients with lymph node metastasis exhibited significantly higher levels of phosphorylated forms of HspB1 in the nucleus and cytoplasm compared with the group without lymph node metastasis. Analysis showed that the expression of phosphorylated forms of the chaperone HspB1 correlates with the amount and percentage of lymph node metastases affected.
The nuclear expression of phosphorylated and non-phosphorylated forms of the chaperone HspB1 is a marker of tumor cells associated with lymphatic metastasis of breast cancer.
热休克蛋白β-1（HspB1）是小分子热休克蛋白（sHsp）的一种伴侣蛋白。sHsp的常见功能包括伴侣活性、抑制细胞凋亡、调节细胞发育和细胞分化，并参与信号转导。
研究原发性乳腺癌细胞中HspB1磷酸化形式和非磷酸化形式的细胞内定位，并评估它们与区域淋巴结转移的关系。
收集100例年龄在31 - 80岁之间确诊为非特异性浸润性癌的患者的乳腺组织肿瘤活检标本。采用免疫组织化学方法确定HspB1磷酸化形式和非磷酸化形式的细胞内定位。
本研究结果显示，与无淋巴结转移组相比，有淋巴结转移患者的活检标本中，细胞核和细胞质中HspB1磷酸化形式的水平显著更高。分析表明，伴侣蛋白HspB1磷酸化形式的表达与受影响的淋巴结转移数量和百分比相关。
伴侣蛋白HspB1磷酸化形式和非磷酸化形式的核表达是与乳腺癌淋巴结转移相关的肿瘤细胞标志物。
