用免疫过氧化物酶技术检测组织中重新激活的伪狂犬病病毒。

Detection of reactivating pseudorabies virus in tissue by immunoperoxidase technique.

作者信息

Narita M, Imada T, Takada M, Ishibashi K, Nemoto F, Haritani M, Kobayashi M

机构信息

National Institute of Animal Health, Ibaraki, Japan.

出版信息

J Comp Pathol. 1989 Aug;101(2):151-9. doi: 10.1016/0021-9975(89)90063-7.

DOI:10.1016/0021-9975(89)90063-7

PMID:2551939

Abstract

Reactivation of pseudorabies virus (PRV) was induced in pigs by prednisolone treatment. The virus was re-isolated from nasal secretions of four, brain cortex of two and mandibular lymph node of three out of 12 pigs, respectively. The characteristic lesion of recurrently infected pigs was focal necrosis in the brain cortex and mandibular lymph node, accompanied by reactivating virus particles in the degenerating cells. Coincident with the pathological lesions, PRV antigen was detected in two of the brain cortex and six of the mandibular lymph node specimens by immunoperoxidase staining. These results suggest that the immunoperoxidase technique was more sensitive than virus isolation for demonstrating the reactivity of PRV in recurrently infected pigs.

摘要

通过泼尼松龙治疗诱导猪体内伪狂犬病病毒（PRV）重新激活。分别从12头猪中的4头的鼻分泌物、2头的脑皮质以及3头的下颌淋巴结中重新分离出该病毒。反复感染猪的特征性病变为脑皮质和下颌淋巴结中的局灶性坏死，并伴有变性细胞中重新激活的病毒颗粒。与病理病变一致，通过免疫过氧化物酶染色在2份脑皮质标本和6份下颌淋巴结标本中检测到PRV抗原。这些结果表明，在证明PRV在反复感染猪中的反应性方面，免疫过氧化物酶技术比病毒分离更敏感。