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[蛋白质和膜中旋转自旋标记及探针的模型]

[Models for rotating spin labels and probes in proteins and membranes].

作者信息

Antsiferova L I, Belonogova O V, Kochetkov V V, Likhtenshteĭn G I

出版信息

Izv Akad Nauk SSSR Biol. 1989 Jul-Aug(4):494-501.

PMID:2551945
Abstract

A method of comparative analysis of ESR spectra has been proposed. It allows to distinguish between two models, slow reorientation of spin labels, and rapid rotation of the cone. Comparison of experimental data for a number of biological objects the theoretical predictions has shown that the rotation of nitroxyl fragment of spin labels can be described by the model of slow anisotropic rotation with correlation time 10(-6) less than or equal to tau less than or equal to 10(-8) s in conditions where the rotation of macromolecules is "frozen".

摘要

提出了一种电子自旋共振(ESR)光谱的比较分析方法。它能够区分两种模型,即自旋标记的缓慢重取向和锥体的快速旋转。对一些生物对象的实验数据与理论预测进行比较表明,在大分子旋转“冻结”的条件下,自旋标记的硝酰基片段的旋转可以用相关时间为10^(-6)≤τ≤10^(-8) s的缓慢各向异性旋转模型来描述。

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