Kumar Kanthasamy Jayabal Senthil, Li Justine, Vani Muthuraj Gokila, Hsieh Yu-Hsin, Kuo Yueh-Hsiung, Wang Sheng-Yang
Department of Forestry, National Chung Hsing University, Taichung, Taiwan.
Department of Neurobiology and Behavior, Department of Psychology, College of Arts and Sciences, Honors College, Stony Brook University, New York, USA.
Planta Med. 2015 Jan;81(1):39-45. doi: 10.1055/s-0034-1383361. Epub 2014 Dec 17.
Formosan sweetgum (Liquidamber formosana) is an endemic tree species. Various parts of this tree are used as a traditional Chinese medicine for treating pain, inflammation, and rheumatic disorders. In this study, we investigated the anti-inflammatory potential of bornyl cinnamate, a cinnamic acid derivative from the essential oil of L. formosana. Pretreatment with bornyl cinnamate significantly inhibited lipopolysaccharide-induced proinflammatory molecules, including nitric oxide, prostaglandin-E2, tumor necrosis factor α, and interleukin-1β production, in murine macrophage RAW 264.7 cells. RT-PCR and immunoblotting analysis revealed that the inhibition of the proinflammatory molecules occurred through the downregulation of their corresponding mediator genes. Immunofluorescence and luciferase reporter assays revealed that the inhibition of proinflammatory genes by bornyl cinnamate was caused by the suppression of nuclear translocation and transcriptional activation of the redox-sensitive transcription factor nuclear factor κB. In addition, bornyl cinnamate increased the protein stability of the inhibitor of nuclear factor κB, an endogenous repressor of nuclear factor κB, through inhibition of its phosphorylation and proteasomal degradation. Furthermore, bornyl cinnamate significantly blocked the lipopolysaccharide-induced activation of I-κB kinase α, an upstream kinase of the inhibitor of nuclear factor κB α. Taken together, these results suggest that bornyl cinnamate could inhibit proinflammatory molecules through the suppression of the redox-sensitive nuclear factor κB signaling pathway.
台湾枫香（Liquidambar formosana）是一种本土树种。该树的各个部位被用作传统中药，用于治疗疼痛、炎症和风湿性疾病。在本研究中，我们研究了肉桂酸冰片酯的抗炎潜力，它是台湾枫香精油中的一种肉桂酸衍生物。在小鼠巨噬细胞RAW 264.7细胞中，用肉桂酸冰片酯预处理可显著抑制脂多糖诱导的促炎分子，包括一氧化氮、前列腺素 - E2、肿瘤坏死因子α和白细胞介素 - 1β的产生。RT - PCR和免疫印迹分析表明，促炎分子的抑制是通过下调其相应的介导基因实现的。免疫荧光和荧光素酶报告基因检测表明，肉桂酸冰片酯对促炎基因的抑制是由氧化还原敏感转录因子核因子κB的核转位和转录激活的抑制引起的。此外，肉桂酸冰片酯通过抑制核因子κB抑制剂（核因子κB的内源性抑制剂）的磷酸化和蛋白酶体降解，增加了其蛋白质稳定性。此外，肉桂酸冰片酯显著阻断了脂多糖诱导的核因子κBα抑制剂上游激酶I - κB激酶α的激活。综上所述，这些结果表明肉桂酸冰片酯可通过抑制氧化还原敏感的核因子κB信号通路来抑制促炎分子。
