利用抗2型马立克氏病病毒单克隆抗体鉴定病毒特异性多肽。

Identification of virus-specific polypeptides by monoclonal antibodies against serotype 2 Marek's disease virus.

作者信息

Nakajima K, Shibayama T, Yokota M, Ikuta K, Kato S, Hirai K

机构信息

Department of Pathology, Osaka University, Japan.

出版信息

J Gen Virol. 1989 Oct;70 ( Pt 10):2563-71. doi: 10.1099/0022-1317-70-10-2563.

DOI:10.1099/0022-1317-70-10-2563

PMID:2552003

Abstract

A total of 41 antibody-secreting hybridoma cells against the HPRS24 strain of Marek's disease virus (MDV) type 2 (MDV2) have been isolated. Of these monoclonal antibodies (MAbs), 24 were found by immunofluorescence tests to react specifically with MDV2-infected cells, but not MDV type 1 (MDV1)- or herpesvirus of turkeys (HVT)-infected cells, while eight reacted with MDV1- or MDV2-infected cells and nine with MDV1-, MDV2- or HVT-infected cells. By using these MAbs, seven classes of MDV type-specific or cross-reactive polypeptides were characterized by immunoprecipitation followed by SDS-PAGE. Among them, a 28K/32K glycoprotein differed from the previously identified gA and gB. The 28K/32K glycoprotein was found on the surface of MDV2-infected cells and in the cytoplasm by an immunofluorescence test with MAbs. In addition, a cross-reactive polypeptide of 25K/29K was also detected in MDV1-infected cells with MAbs reactive with the 28K/32K glycoprotein of MDV2.

摘要

共分离出41株针对2型马立克氏病病毒（MDV2）HPRS24毒株的分泌抗体杂交瘤细胞。在这些单克隆抗体（MAb）中，通过免疫荧光试验发现24株与MDV2感染细胞特异性反应，但不与1型马立克氏病病毒（MDV1）或火鸡疱疹病毒（HVT）感染细胞反应，8株与MDV1或MDV2感染细胞反应，9株与MDV1、MDV2或HVT感染细胞反应。利用这些单克隆抗体，通过免疫沉淀后进行SDS-PAGE，鉴定出7类MDV型特异性或交叉反应性多肽。其中，一种28K/32K糖蛋白与先前鉴定的gA和gB不同。通过用单克隆抗体进行免疫荧光试验，在MDV2感染细胞的表面和细胞质中发现了28K/32K糖蛋白。此外，在MDV1感染细胞中也检测到一种与MDV2的28K/32K糖蛋白反应的单克隆抗体的25K/29K交叉反应性多肽。