Kitazawa T, Ono M, Maeda K, Kawaguchi Y, Kamiya N, Niikura M, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
J Vet Med Sci. 1993 Dec;55(6):985-90. doi: 10.1292/jvms.55.985.
The sequence analysis of the genomic region of Marek's disease virus (MDV) serotype 2 (MDV2) which had homology with the gene encoding MDV serotype 1 (MDV1) homologue of glycoprotein C (gC) of herpes simplex virus revealed that there was a single open reading frame (ORF) capable of encoding 479 amino acids. The predicted protein from this ORF had strong hydrophobic regions in both the amino and carboxyl termini and several potential N-linked glycosylation sites, as seen in those from the ORFs of MDV1 and MDV serotype 3 (MDV3 = herpesvirus of turkeys (HVT)) gC homologues. By northern-blot analysis using a DNA fragment located in the MDV2 gC homologue ORF as a probe, 3.6 and 1.9 kb RNA transcripts were identified in MDV2-infected cells. When the alignment of amino acid sequences of the gC homologous genes of three MDV serotypes were compared, the homology between MDV1 and MDV2 and that between MDV1 and HVT showed about 76% identity, whereas that between MDV2 and HVT was about 70% identity. These results suggested a possibility that the evolutionary distance between MDV1 and MDV2 and between MDV1 and HVT is shorter than that between MDV2 and HVT.
对2型马立克氏病病毒（MDV2）基因组区域进行序列分析，该区域与编码单纯疱疹病毒糖蛋白C（gC）的1型马立克氏病病毒（MDV1）同源基因具有同源性，结果显示存在一个单一的开放阅读框（ORF），能够编码479个氨基酸。该ORF预测的蛋白质在氨基和羧基末端均有强疏水区域以及几个潜在的N-连接糖基化位点，这与MDV1和3型马立克氏病病毒（MDV3 = 火鸡疱疹病毒（HVT））gC同源物的ORF所预测的蛋白质情况相同。通过使用位于MDV2 gC同源物ORF中的DNA片段作为探针进行Northern杂交分析，在MDV2感染的细胞中鉴定出了3.6和1.9 kb的RNA转录本。当比较三种MDV血清型gC同源基因的氨基酸序列比对时，MDV1与MDV2之间以及MDV1与HVT之间的同源性显示约76%的一致性，而MDV2与HVT之间的同源性约为70%。这些结果表明，MDV1与MDV2之间以及MDV1与HVT之间的进化距离可能比MDV2与HVT之间的进化距离短。
