分泌肿瘤坏死因子的人胚肾细胞对人慢性淋巴细胞白血病中B淋巴细胞凋亡的影响

Effects of TNF secreting HEK cells on B lymphocytes' apoptosis in human chronic lymphocytic leukemias.

作者信息

Valizadeh Armita, Ahmadzadeh Ahmad, Teimoori Ali, Khodadadi Ali, Saki Ghasem

机构信息

Department of Anatomy, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran E-mail :

出版信息

Asian Pac J Cancer Prev. 2014;15(22):9885-9. doi: 10.7314/apjcp.2014.15.22.9885.

DOI:10.7314/apjcp.2014.15.22.9885

PMID:25520123

Abstract

BACKGROUND

Tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL) is an antitumor candidate in cancer therapy. This study focused on effects of TRAIL, as a proapototic ligand that causes apoptosis, in B-CELL chronic lymphocytic leukemia cells (B-CLL) .

MATERIALS AND METHODS

A population of HEK 293 cells was transducted by lentivirus that these achieved ability for producing the TRAIL protein and then HEK 293 cells transducted were placed in the vicinity of CLL cells. After 24 hours of co-culture, apoptosis of CLL cells was assessed by annexin V staining.

RESULTS

The amount of Apoptosis was examined separately in four groups: 293 HEK TRAIL (16.17±1.04%); 293 HEK GFP (2.7±0.57%); WT 293 HEK (2±2.6%); and CLL cells (0.01±0.01%). Among the groups studied, the maximum amount of apoptosis was in the group that the vector encoding TRAIL was transducted. In this group, the mean level of soluble TRAIL in the culture medium was 253 pg/ml; also flow cytometry analyzes showed that proapotosis in this group was 32.8±1.6%, which was higher than the other groups.

CONCLUSIONS

In this study, we have demonstrated that TNF secreted from HEK 293 cells are effective in death of CLL cells.

摘要

背景

肿瘤坏死因子（TNF）相关凋亡诱导配体（TRAIL）是癌症治疗中的一种抗肿瘤候选物。本研究聚焦于TRAIL作为一种促凋亡配体在B细胞慢性淋巴细胞白血病细胞（B-CLL）中诱导凋亡的作用。

材料与方法

用慢病毒转导一群HEK 293细胞，使其获得产生TRAIL蛋白的能力，然后将转导后的HEK 293细胞置于CLL细胞附近。共培养24小时后，通过膜联蛋白V染色评估CLL细胞的凋亡情况。

结果

分别在四组中检测凋亡量：293 HEK TRAIL组（16.17±1.04%）；293 HEK GFP组（2.7±0.57%）；野生型293 HEK组（2±2.6%）；以及CLL细胞组（0.01±0.01%）。在所研究的组中，凋亡量最大的是转导了编码TRAIL载体的组。在该组中，培养基中可溶性TRAIL的平均水平为253 pg/ml；流式细胞术分析还显示该组的促凋亡率为32.8±1.6%，高于其他组。