使用干血斑进行HIV病毒载量检测的外部质量评估计划的设计与实施
Design and implementation of an external quality assessment program for HIV viral load measurements using dried blood spots.
作者信息
Prach Lisa M, Puren Adrian, Lippman Sheri A, Carmona Sergio, Stephenson Sophie, Cutler Ewalde, Barnhart Scott, Liegler Teri
机构信息
University of California San Francisco, San Francisco, California, USA.
National Institute for Communicable Diseases/NHLS, Johannesburg, South Africa University of Witwatersrand, Johannesburg, South Africa.
出版信息
J Clin Microbiol. 2015 Mar;53(3):964-6. doi: 10.1128/JCM.02698-14. Epub 2014 Dec 17.
Abstract
An external quality assurance program was developed for HIV-1 RNA viral load measurements taken from dried blood spots using a reference panel and field-collected specimens. The program demonstrated that accurate and reproducible quantitation can be obtained from field-collected specimens. Residual proviral DNA may confound interpretation in virologically suppressed subjects.
摘要
针对使用参考样本和现场采集的标本对干血斑进行的HIV-1 RNA病毒载量测量，制定了一项外部质量保证计划。该计划表明，从现场采集的标本中可获得准确且可重复的定量结果。残留的前病毒DNA可能会混淆病毒学抑制患者的检测结果解读。
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