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使用干血斑进行HIV病毒载量检测的外部质量评估计划的设计与实施

Design and implementation of an external quality assessment program for HIV viral load measurements using dried blood spots.

作者信息

Prach Lisa M, Puren Adrian, Lippman Sheri A, Carmona Sergio, Stephenson Sophie, Cutler Ewalde, Barnhart Scott, Liegler Teri

机构信息

University of California San Francisco, San Francisco, California, USA.

National Institute for Communicable Diseases/NHLS, Johannesburg, South Africa University of Witwatersrand, Johannesburg, South Africa.

出版信息

J Clin Microbiol. 2015 Mar;53(3):964-6. doi: 10.1128/JCM.02698-14. Epub 2014 Dec 17.

DOI:10.1128/JCM.02698-14
PMID:25520449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4390657/
Abstract

An external quality assurance program was developed for HIV-1 RNA viral load measurements taken from dried blood spots using a reference panel and field-collected specimens. The program demonstrated that accurate and reproducible quantitation can be obtained from field-collected specimens. Residual proviral DNA may confound interpretation in virologically suppressed subjects.

摘要

针对使用参考样本和现场采集的标本对干血斑进行的HIV-1 RNA病毒载量测量，制定了一项外部质量保证计划。该计划表明，从现场采集的标本中可获得准确且可重复的定量结果。残留的前病毒DNA可能会混淆病毒学抑制患者的检测结果解读。

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本文引用的文献

1
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2
Informing comprehensive HIV prevention: a situational analysis of the HIV prevention and care context, North West Province South Africa.为全面的艾滋病病毒预防工作提供信息：南非西北省艾滋病病毒预防与护理情况分析
PLoS One. 2014 Jul 16;9(7):e102904. doi: 10.1371/journal.pone.0102904. eCollection 2014.
3
Assessment of population-based HIV RNA levels in a rural east African setting using a fingerprick-based blood collection method.基于指尖采血法评估东非农村地区的人群 HIV RNA 水平。
Clin Infect Dis. 2013 Feb;56(4):598-605. doi: 10.1093/cid/cis881. Epub 2012 Dec 12.
4
Current and future applications of dried blood spots in viral disease management.干血斑在病毒病管理中的当前和未来应用。
Antiviral Res. 2012 Mar;93(3):309-21. doi: 10.1016/j.antiviral.2011.12.011. Epub 2012 Jan 5.
5
Utility of routine viral load, CD4 cell count, and clinical monitoring among adults with HIV receiving antiretroviral therapy in Uganda: randomised trial.在乌干达接受抗逆转录病毒治疗的艾滋病毒感染者中，常规病毒载量、CD4 细胞计数和临床监测的效用：随机试验。
BMJ. 2011 Nov 9;343:d6792. doi: 10.1136/bmj.d6792.
6
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J Clin Microbiol. 2009 Apr;47(4):1107-18. doi: 10.1128/JCM.02255-08. Epub 2009 Feb 4.