大鼠重复给予柚皮苷后柚皮素共轭代谢物的组织分布情况。
Tissue distribution of naringenin conjugated metabolites following repeated dosing of naringin to rats.
作者信息
Lin Shiuan-Pey, Hou Yu-Chi, Tsai Shang-Yuan, Wang Meng-Ju, Chao Pei-Dawn Lee
机构信息
School of Pharmacy, China Medical University, No.91 Hsueh-Shih Road, Taichung, Taiwan.
School of Pharmacy, China Medical University, No.91 Hsueh-Shih Road, Taichung, Taiwan ; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan ; Department of Pharmacy, China Medical University hospital, Taichung, Taiwan.
出版信息
Biomedicine (Taipei). 2014;4(3):16. doi: 10.7603/s40681-014-0016-z. Epub 2014 Aug 13.
Background: Naringin is a major antioxidant in fruits and herbs. To clarify molecular forms distributed to various tissues, we investigated tissue distribution of naringin and relevant metabolites in rats after repeated dosing. Methods: Male Sprague-Dawley rats were orally administered naringin (210 mg/kg) twice daily for eight days. At 6 h post the 17 dose, various tissues including liver, kidney, heart, spleen and brain were collected and analyzed by HPLC method before and after hydrolysis with β-glucuronidase and sulfatase, individually. Results: The free forms of naringin and naringenin were not detected in all the tissues assayed. Liver contained the highest concentration of naringenin sulfates, followed by spleen, heart, brain and kidney. Naringenin glucuronides were present in liver and kidney, but not in spleen, brain and heart. Conclusion: The bioavailability of naringenin glucuronides and sulfates supported its application for personalized medicine.
背景
柚皮苷是水果和草药中的主要抗氧化剂。为了阐明分布到各种组织中的分子形式，我们研究了重复给药后大鼠体内柚皮苷及其相关代谢物的组织分布。方法：雄性Sprague-Dawley大鼠每天口服两次柚皮苷（210mg/kg），持续八天。在第17次给药后6小时，收集包括肝脏、肾脏、心脏、脾脏和大脑在内的各种组织，分别用β-葡萄糖醛酸酶和硫酸酯酶水解前后，通过HPLC法进行分析。结果：在所检测的所有组织中均未检测到柚皮苷和柚皮素的游离形式。肝脏中柚皮素硫酸盐的浓度最高，其次是脾脏、心脏、大脑和肾脏。柚皮素葡萄糖醛酸苷存在于肝脏和肾脏中，但不存在于脾脏、大脑和心脏中。结论：柚皮素葡萄糖醛酸苷和硫酸盐的生物利用度支持其在个性化医学中的应用。
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