Zhang Yong, Cheng Yan, Guo Jiahui, Yang Ennian, Liu Cheng, Zheng Xuelian, Deng Kejun, Zhou Jianping
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Int J Mol Sci. 2014 Dec 16;15(12):23332-44. doi: 10.3390/ijms151223332.
Wheat hybrid necrosis is an interesting genetic phenomenon that is found frequently and results in gradual death or loss of productivity of wheat. However, the molecular basis and mechanisms of this genetic phenomenon are still not well understood. In this study, the transcriptomes of wheat hybrid necrosis F1 and its parents (Neimai 8 and II469) were investigated using digital gene expression (DGE). A total of 1300 differentially expressed genes were identified, indicating that the response to hybrid necrosis in wheat is complicated. The assignments of the annotated genes based on Gene Ontology (GO) revealed that most of the up-regulated genes belong to "universal stress related", "DNA/RNA binding", "protein degradation" functional groups, while the down-regulated genes belong to "carbohydrate metabolism" and "translation regulation" functional groups. These findings suggest that these pathways were affected by hybrid necrosis. Our results provide preliminarily new insight into the underlying molecular mechanisms of hybrid necrosis and will help to identify important candidate genes involved in wheat hybrid necrosis.
小麦杂种坏死是一种常见的有趣遗传现象，会导致小麦逐渐死亡或生产力丧失。然而，这种遗传现象的分子基础和机制仍未得到充分理解。在本研究中，利用数字基因表达（DGE）技术对小麦杂种坏死F1及其亲本（内麦8号和II469）的转录组进行了研究。共鉴定出1300个差异表达基因，表明小麦对杂种坏死的反应较为复杂。基于基因本体论（GO）对注释基因的分类显示，大多数上调基因属于“普遍应激相关”、“DNA/RNA结合”、“蛋白质降解”功能组，而下调基因属于“碳水化合物代谢”和“翻译调控”功能组。这些发现表明这些途径受到杂种坏死的影响。我们的结果为杂种坏死的潜在分子机制提供了初步的新见解，并将有助于鉴定参与小麦杂种坏死的重要候选基因。
