Kubik Grzegorz, Summerer Daniel
Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany).
Chembiochem. 2015 Jan 19;16(2):228-31. doi: 10.1002/cbic.201402408. Epub 2014 Dec 17.
We report engineered transcription-activator-like effectors (TALEs) as the first DNA-binding molecules that detect 5-methylcytosine (mC) at single-nucleotide resolution with fully programmable sequence selectivity. This is achieved by a design strategy such that a single cytosine (C) in a DNA sequence is selectively interrogated for its mC-modification level by targeting with a discriminatory TALE repeat; other Cs are ignored by targeting with universal-binding TALE repeats.
我们报告了工程化转录激活样效应因子（TALEs），这是首批能够以完全可编程的序列选择性在单核苷酸分辨率下检测5-甲基胞嘧啶（mC）的DNA结合分子。这是通过一种设计策略实现的，即通过用具有鉴别性的TALE重复序列靶向，对DNA序列中的单个胞嘧啶（C）的mC修饰水平进行选择性询问；其他的C则通过用通用结合TALE重复序列靶向而被忽略。
