Moore Richard, Chandrahas Anita, Bleris Leonidas
Bioengineering Department, The University of Texas at Dallas , 800 West Campbell Road, Richardson, Texas 75080 United States.
ACS Synth Biol. 2014 Oct 17;3(10):708-16. doi: 10.1021/sb400137b. Epub 2014 Feb 13.
Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which determines the DNA targeting specificity and can be rapidly synthesized de novo. Considering the highly modular nature of TALEs, their versatility, and the ease of constructing these proteins, this technology can have important implications for synthetic biology applications. Here, we review developments in the area with a particular focus on modifications for custom and controllable gene regulation.
转录激活样效应因子（TALEs）是由黄单胞菌分泌的蛋白质，用于辅助感染植物物种。TALEs通过与特定DNA序列结合并激活宿主基因的表达来协助感染。最近的研究结果表明，TALE蛋白由一个中央重复结构域组成，该结构域决定了DNA靶向特异性，并且可以从头快速合成。鉴于TALEs的高度模块化性质、多功能性以及构建这些蛋白质的简易性，这项技术可能对合成生物学应用具有重要意义。在这里，我们回顾该领域的进展，特别关注用于定制和可控基因调控的修饰。
