Ojha Abhishek, Sree K Sowjanya, Sachdev Bindiya, Rashmi M A, Ravi K C, Suresh P J, Mohan Komarlingam S, Bhatnagar Raj K
a International Centre For Genetic Engineering and Biotechnology ; New Delhi , India.
GM Crops Food. 2014;5(4):280-6. doi: 10.4161/21645698.2014.947800.
High survivorship of pink bollworrm, Pectinophora gossypiella in bolls of Bollgard® cotton hybrids and resistance to Cry1Ac protein, expressed in Bollgard cotton were reported in field-populations collected from the state of Gujarat (western India) in 2010. We have found Cry1Ac-resistance in pink bollworm populations sourced from Bollgard and non-Bt cotton fields in the adjoining states of Maharashtra and Madhya Pradesh in Central India. Further, we observed reduced binding of labeled Cry1Ac protein to receptors localized on the brush-border membrane of pink bollworm larval strains with high tolerance to Cry1Ac. These strains were sourced from Bollgard and conventional cotton fields. A pooled Cry1Ac-resistant strain, further selected on Cry1Ac diet also showed significantly reduced binding to Cry1Ac protein. The reduced binding of Cry1Ac to receptors could be an underlying mechanism for the observed resistance in pink bollworm populations feeding on Bollgard hybrids.
2010年，在从印度西部古吉拉特邦收集的田间种群中，报道了棉铃虫在Bollgard®棉花杂交种棉铃中的高存活率以及对Bollgard棉花中表达的Cry1Ac蛋白的抗性。我们在印度中部毗邻的马哈拉施特拉邦和中央邦的Bollgard棉田和非转基因抗虫棉田中发现了棉铃虫对Cry1Ac的抗性。此外，我们观察到，对Cry1Ac具有高耐受性的棉铃虫幼虫品系，其刷状缘膜上定位的受体与标记的Cry1Ac蛋白的结合减少。这些品系来源于Bollgard棉田和常规棉田。在Cry1Ac饲料上进一步筛选出的一个混合的抗Cry1Ac品系，对Cry1Ac蛋白的结合也显著减少。Cry1Ac与受体结合的减少可能是取食Bollgard杂交种的棉铃虫种群中观察到的抗性的潜在机制。
