Rowell N P, McCready V R, Cronin B, Pepper J, Higley B, Burke J F, Tyrrell D A
Department of Radiotherapy, Royal Marsden Hospital, Sutton, Surrey, UK.
Nucl Med Commun. 1989 Jul;10(7):503-8. doi: 10.1097/00006231-198907000-00009.
Levels of reduced glutathione (GSH) are increased in some types of malignant tumours and are known to influence the response to radio- and chemotherapy. In vitro studies suggest a correlation between cellular GSH concentration and retention of the meso form of hexamethyl propyleneamineoxime (HMPAO). This study investigates the relationship between in vivo tissue retention of 99Tcm-labelled HMPAO and GSH concentration in ten patients referred for thoracotomy for possible lung cancer. Retention of 99Tcm-HMPAO was measured using single photon emission computed tomography (SPECT). The tumour and normal lung concentration of reduced glutathione (GSH) was measured in tissue specimens collected peroperatively. Malignancy was confirmed in eight patients. Of seven patients undergoing curative resection for carcinoma, tumour GSH concentration was higher (mean 2.76 mM) than normal lung (mean 1.04 mM). In one neurofibroma, the GSH concentration was 1.80 mM. No correlation was found between 99Tcm meso-HMPAO retention and either the tumour GSH concentration or the tumour:lung GSH ratio. The results from this small series demonstrate that the intracellular GSH concentration of malignant lung tumours is generally higher than that in normal lung but that meso-HMPAO retention could not be used to predict these levels.
在某些类型的恶性肿瘤中，还原型谷胱甘肽（GSH）水平会升高，并且已知其会影响对放疗和化疗的反应。体外研究表明细胞内GSH浓度与六甲基丙烯胺肟（HMPAO）的内消旋形式的滞留之间存在相关性。本研究调查了10例因可能的肺癌而接受开胸手术的患者体内99Tcm标记的HMPAO组织滞留与GSH浓度之间的关系。使用单光子发射计算机断层扫描（SPECT）测量99Tcm-HMPAO的滞留情况。在手术中收集的组织标本中测量还原型谷胱甘肽（GSH）的肿瘤和正常肺浓度。8例患者确诊为恶性肿瘤。在7例接受根治性癌切除术的患者中，肿瘤GSH浓度（平均2.76 mM）高于正常肺（平均1.04 mM）。在1例神经纤维瘤中，GSH浓度为1.80 mM。未发现99Tcm内消旋-HMPAO滞留与肿瘤GSH浓度或肿瘤：肺GSH比值之间存在相关性。这个小系列的结果表明，恶性肺肿瘤的细胞内GSH浓度通常高于正常肺，但内消旋-HMPAO滞留不能用于预测这些水平。
