来自大肠杆菌的天然乳糖抑制蛋白（LacI）比带有LVA标签的LacI产生更快的反应和更高水平的表达。

Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI.

作者信息

Andreassen Patrick Rosendahl, Fredberg Sofie, Horan Mattias, Knudsen Maria Højberg, Jacobsen Kirstine, Kjær Andreas, Krogh Thøger Jensen, Kronborg Tina, Mattsson Nicky Cordua, Schmidt Sissel Ida, Wille Heidi, Andersen Ann

机构信息

Department of Biochemistry and Molecular Biology, ‡Department of Cancer & Inflammation Research, §Department of Mathematics and Computer Science, ∥SDU Business, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.

出版信息

ACS Synth Biol. 2014 Dec 19;3(12):949-52. doi: 10.1021/sb500031h.

DOI:10.1021/sb500031h

PMID:25524095

Abstract

The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

摘要

乳糖启动子是大肠杆菌中用于重组基因表达控制的最常用启动子之一。在没有半乳糖苷的情况下，乳糖启动子被其阻遏蛋白LacI抑制。由于乳糖启动子受阻遏物调控，当该启动子导入高拷贝质粒时，LacI的过表达对于调控是必要的。为此，LacI的一种修饰变体，即带有LVA标签的LacI，已提交至标准生物部件登记库，自那时起已被用于500多个构建体中。然而，我们发现，天然LacI作为表达控制器优于带有LVA标签的LacI。