Nasehi Mohammad, Tabatabaie Maryam, Khakpai Fatemeh, Zarrindast Mohammad-Reza
Medical Genomics Research Center and School of Advanced Sciences in Medicine, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
Institute for Cognitive Science Studies (ICSS), Tehran, Iran.
Neurosci Lett. 2015 Feb 5;587:73-8. doi: 10.1016/j.neulet.2014.12.019. Epub 2014 Dec 15.
In this study, the effects of 5-HT4 receptors of the CA1 on MK801-induced amnesia and hyperlocomotion were examined. One-trial step-down method was used to assess memory retention and then, the hole-board method to assess exploratory behaviors. The results showed that post-training intra-CA1 administration of RS67333 (62.5 and 625 ng/mouse) and RS23597 (1 and 10 ng/mouse) decreased memory consolidation, but it did not alter head-dip counts, head-dip latency and locomotor activity. Similarly, MK801 (0.5 and 1 μg/mouse) decreased memory consolidation, but had no effect on head-dip counts and head-dip latency. Interestingly, it increased locomotor activity. The results also showed that post-training intra-CA1 injection of a sub-threshold dose of RS67333 (6.25 ng/mouse) or RS23597 (0.1 ng/mouse) could heighten MK801 induced amnesia and decrease locomotor activity, but it did not alter head-dip counts and head-dip latency. In conclusion, our findings suggest that the CA1 5-HT4 receptors are involved in MK801-induced amnesia and hyperlocomotion.
在本研究中，检测了CA1区5 - HT4受体对MK801诱导的失忆和运动亢进的影响。采用一次性被动回避法评估记忆保持，然后用洞板法评估探索行为。结果显示，训练后向CA1区内注射RS67333（62.5和625 ng/只小鼠）和RS23597（1和10 ng/只小鼠）会降低记忆巩固，但不改变探洞次数、探洞潜伏期和运动活性。同样，MK801（0.5和1 μg/只小鼠）降低记忆巩固，但对探洞次数和探洞潜伏期无影响。有趣的是，它会增加运动活性。结果还显示，训练后向CA1区内注射亚阈值剂量的RS67333（6.25 ng/只小鼠）或RS23597（0.1 ng/只小鼠）可增强MK801诱导的失忆并降低运动活性，但不改变探洞次数和探洞潜伏期。总之，我们的研究结果表明，CA1区5 - HT4受体参与了MK801诱导的失忆和运动亢进。
