Pérez-Sayáns Mario, Suárez-Peñaranda José Manuel, Iruegas Elena Padín, de Almeida Miguel Reis, Barros-Angueira Francisco, Torreira Mercedes Gallas, García-García Abel
Oral Surgery and Implantology Unit, Faculty of Medicine and Dentistry, Instituto de Investigación Sanitaria de Santiago, Santiago de Compostela, Spain.
Department of Pathology and Forensic Sciences, University Hospital and School of Medicine, Santiago de Compostela, Spain.
Cancer Biomark. 2015;15(1):19-26. doi: 10.3233/CBM-140440.
Downregulation of p21{Waf1/CIP1} (a cyclin-dependent kinase inhibitor) has been reported for mouth cancer. The goal of this article is to quantitatively report expression of p21{Waf1/CIP1} and evaluate its relationship with the clinical and prognostic factors.
this is a retrospective study of 68 patients diagnosed with OSCC. We constructed a tissue microarray to develop an immunohistochemical assessment of p21{Waf1/CIP1} expression. A multivariate analysis using a forward-selection stepwise regression model (Cox, 1972) for predicting survival was performed.
The quantitative expression of p21{Waf1/CIP1} showed a statistically significant relationship with the risk of lymph node metastasis, showing a higher expression in patients with homolateral single nodes of less than 3 cm (N1) (X{2}=6.58; p< 0.05). We found no statistically significant relationship with any other clinical or pathological parameters. The Cox univariate regression analysis verifies that the effect of the value of p21{Waf1/CIP1} on survival was not statistically significant (p=0.6). The best predictive multivariate Cox analysis included the covariates: recurrence, p21{Waf1/CIP1}, gender, stage, and dysplasia in the adjacent margin. All these variables showed a statistically significant relationship with survival, except p21{Waf1/CIP1}.
quantitative determination of p21{Waf1/CIP1} standardizes and facilitates its analysis. Although its expression increases in patients with N1 regional metastasis, the loss of p21{WAF1/CIP1} does not seem to have any relationship with the clinical and pathological variables of the tumors.
已有报道称口腔癌中p21{Waf1/CIP1}（一种细胞周期蛋白依赖性激酶抑制剂）表达下调。本文旨在定量报告p21{Waf1/CIP1}的表达情况，并评估其与临床及预后因素的关系。
这是一项对68例诊断为口腔鳞状细胞癌（OSCC）患者的回顾性研究。我们构建了组织芯片，以开展p21{Waf1/CIP1}表达的免疫组化评估。采用向前选择逐步回归模型（Cox，1972）进行多因素分析以预测生存率。
p21{Waf1/CIP1}的定量表达与淋巴结转移风险呈统计学显著相关，在同侧单个淋巴结小于3 cm（N1）的患者中表达较高（X{2}=6.58；p<0.05）。我们发现其与任何其他临床或病理参数均无统计学显著关系。Cox单因素回归分析证实p21{Waf1/CIP1}值对生存的影响无统计学显著性（p=0.6）。最佳预测多因素Cox分析纳入的协变量有：复发、p21{Waf1/CIP1}、性别、分期及切缘邻近组织发育异常。除p21{Waf1/CIP1}外，所有这些变量均与生存呈统计学显著关系。
p21{Waf1/CIP1}的定量测定使其分析标准化且更便利。虽然在N1区域转移患者中其表达增加，但p21{WAF1/CIP1}的缺失似乎与肿瘤的临床和病理变量无任何关系。
