Bielli Mattia, Nardini Giordano, Di Girolamo Nicola, Savarino Paolo
Private practice Michelangelo, Novara, Italy (Bielli)Veterinary Clinic Modena Sud, Spilamberto, Modena, Italy (Nardini)Clinic for Exotic Animals, Centro Veterinario Specialistico, Rome, Italy (Di Girolamo)Private practitioner, Turin, Italy (Savarino)
Private practice Michelangelo, Novara, Italy (Bielli)Veterinary Clinic Modena Sud, Spilamberto, Modena, Italy (Nardini)Clinic for Exotic Animals, Centro Veterinario Specialistico, Rome, Italy (Di Girolamo)Private practitioner, Turin, Italy (Savarino).
J Vet Diagn Invest. 2015 Jan;27(1):68-73. doi: 10.1177/1040638714561251.
Hermann's tortoise (Testudo hermanni) is considered near threatened in the wild but, by contrast, it is one of the most popular pet tortoises in Europe. Scant data is reported in the veterinary literature on hematological values for T. hermanni and, to our knowledge, none focused on the subspecies boettgeri (eastern Hermann's tortoise). Published reports are based on small sample populations, and confusion arises when comparing the sampling sites, the anticoagulants, and the counting methods used. The purpose of the current study was to establish the normal mean values and reference intervals for the main hematological parameters for captive adult T. hermanni boettgeri and to evaluate the reliability of a semiautomated blood analyzer for red blood cell count and hematocrit determination. Blood values were determined in 23 adult tortoises using a Neubauer chamber with Natt and Herrick solution; red blood cells and hematocrit were also measured using a semiautomated blood analyzer. Statistical analysis included descriptive statistics, differences between sexes, and agreement between the counting methods. Reference intervals were calculated with the robust method. Wilcoxon signed rank test with continuity correction was used to investigate differences between sexes, and Bland-Altman analysis was performed to compare manual versus semiautomated values. Red blood cells, hematocrit, and hemoglobin determinations were significantly higher in males than in females. White blood cell counts did not show any sex variability. The agreement of manual versus semiautomated determination was considered acceptable for clinical use.
赫尔曼陆龟（Testudo hermanni）在野外被视为近危物种，但相比之下，它是欧洲最受欢迎的宠物龟之一。兽医文献中关于赫尔曼陆龟血液学值的报道很少，据我们所知，没有一项研究聚焦于博氏亚种（东部赫尔曼陆龟）。已发表的报告基于小样本群体，在比较采样地点、抗凝剂和所使用的计数方法时会产生混淆。本研究的目的是确定圈养成年博氏赫尔曼陆龟主要血液学参数的正常平均值和参考区间，并评估半自动血液分析仪用于红细胞计数和血细胞比容测定的可靠性。使用带有纳特和赫里克溶液的纽鲍尔计数板对23只成年陆龟进行血液值测定；还使用半自动血液分析仪测量红细胞和血细胞比容。统计分析包括描述性统计、性别差异以及计数方法之间的一致性。参考区间采用稳健方法计算。使用带连续性校正的威尔科克森符号秩检验来研究性别差异，并进行布兰德-奥特曼分析以比较手动与半自动测量值。雄性的红细胞、血细胞比容和血红蛋白测定值显著高于雌性。白细胞计数未显示出任何性别差异。手动与半自动测定的一致性在临床应用中被认为是可接受的。
