Murray I, Sniderman A D, Cianflone K
Mike Rosenbloom Laboratory for Cardiovascular Research, McGill University Health Centre, Montreal, Quebec, Canada H3A 1A1.
Am J Physiol. 1999 Sep;277(3):E474-80. doi: 10.1152/ajpendo.1999.277.3.E474.
Acylation stimulating protein (ASP), a novel adipocyte-derived autocrine protein, stimulates triglyceride synthesis and glucose transport in vitro in human and murine adipocytes. In vitro, chylomicrons increase ASP and precursor complement C3 production in adipocytes. Furthermore, in vivo, ASP production from human adipose tissue correlates positively with triglyceride clearance postprandially. The aim of the present study was to determine if intraperitoneally injected ASP accelerated triglyceride clearance in vivo after a fat load in C57Bl/6 mice. ASP increased the triglyceride clearance with a reduction of the triglyceride area under the curve over 6 h (AUC(0-6)) from 102.6 +/- 30.0 to 61.0 +/- 14.5 mg. dl(-1). h(-1) (P < 0.05), especially in the latter postprandial period (AUC(3-6); 56.2 +/- 18.0 vs. 24.9 +/- 8.9 mg. dl(-1). h(-1), P < 0.025). ASP also reduced plasma glucose both in the mice with accelerated plasma triglyceride clearance and in those with relatively delayed triglyceride clearance (P < 0.025). Therefore, ASP alters postprandial triglyceride and glucose metabolism.
酰化刺激蛋白（ASP）是一种新发现的源自脂肪细胞的自分泌蛋白，在体外可刺激人和小鼠脂肪细胞内甘油三酯的合成及葡萄糖的转运。在体外，乳糜微粒可增加脂肪细胞中ASP及前体补体C3的生成。此外，在体内，人体脂肪组织产生的ASP与餐后甘油三酯清除呈正相关。本研究的目的是确定腹腔注射ASP是否能加速C57Bl/6小鼠脂肪负荷后体内甘油三酯的清除。ASP增加了甘油三酯清除率，使6小时内甘油三酯曲线下面积（AUC(0 - 6)）从102.6±30.0降至61.0±14.5 mg·dl(-1)·h(-1)（P < 0.05），尤其是在餐后后期（AUC(3 - 6)；56.2±18.0 vs. 24.9±8.9 mg·dl(-1)·h(-1)，P < 0.025）。ASP还降低了血浆甘油三酯清除加速小鼠及甘油三酯清除相对延迟小鼠的血糖水平（P < 0.025）。因此，ASP可改变餐后甘油三酯和葡萄糖代谢。
