Barberio M D, Elmer D J, Laird R H, Lee K A, Gladden B, Pascoe D D
Children's National Medical Center, Center for Genetic Medicine Research, Washington, D.C., United States.
Department of Kinesiology, Berry College, Mount Berry, GA, United States.
Int J Sports Med. 2015 Mar;36(3):262-70. doi: 10.1055/s-0034-1389904. Epub 2014 Dec 19.
This investigation studied circulating LPS activity, potential intestinal damage, and the systemic inflammatory response (SIR) during the exercise heat acclimation process. 8 healthy males (Age=24±3 years) ran in a hot environment on 5 consecutive days until core temperature (Tc) was elevated 2°C above rest. Plasma was obtained pre-, post-, 1 h post-, and 3 h post-exercise on the 1(st), 3(rd), and 5(th) day of exercise and analyzed for TNF-α, IL-6, IL-10, IL-1ra, LPS, and intestinal fatty acid-binding protein (I-FABP). Plasma LPS (1.1 EU·ml(-1)±0.1 vs. 0.7 EU·ml(-1)±0.03; P<0.01) and I-FABP (930.7 pg·ml(-1)±149.0 vs. 640.2 pg·ml(-1)±125.0; P<0.001) were significantly increased post-exercise each. The SIR remained largely unchanged during the study except for TNF-α. Plasma TNF-α was significantly lower on Day 5 at 1 h (3.2 pg·ml(-1)±0.6 vs. 4.5 pg·ml(-1)±0.8; P=0.01) and 3 h (3.6 pg·ml(-1)±0.8 vs. 4.8 pg·ml(-1)±0.9; P=0.05) post-exercise as compared to Day 1. Findings indicate that adaptations to exercise in the heat resulting in reductions of intestinal damage and plasma LPS activity require longer time periods in moderately trained males.
本研究调查了运动热适应过程中循环脂多糖（LPS）活性、潜在的肠道损伤及全身炎症反应（SIR）。8名健康男性（年龄=24±3岁）在炎热环境中连续5天跑步，直至核心体温（Tc）比静息时升高2°C。在运动第1、3、5天运动前、运动后、运动后1小时和3小时采集血浆，分析其中的肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、白细胞介素-1受体拮抗剂（IL-1ra）、LPS和肠脂肪酸结合蛋白（I-FABP）。运动后血浆LPS（1.1 EU·ml⁻¹±0.1 vs. 0.7 EU·ml⁻¹±0.03；P<0.01）和I-FABP（930.7 pg·ml⁻¹±149.0 vs. 640.2 pg·ml⁻¹±125.0；P<0.001）均显著升高。除TNF-α外，研究期间SIR基本保持不变。与第1天相比，第5天运动后1小时（3.2 pg·ml⁻¹±0.6 vs. 4.5 pg·ml⁻¹±0.8；P=0.01）和3小时（3.6 pg·ml⁻¹±0.8 vs. 4.8 pg·ml⁻¹±0.9；P=0.05）时血浆TNF-α显著降低。研究结果表明，对于适度训练的男性，适应热环境运动以减少肠道损伤和血浆LPS活性需要更长时间。
