Shen Hong-Liang, Liu Qing-Jun, Yang Pei-Qian, Tian Ye
Department of Urology, Beijing Friendship Hospital, Capital Medical University, 95 Yong'an Road, Xicheng District, Beijing, 100050, China.
Tumour Biol. 2015 May;36(5):3417-22. doi: 10.1007/s13277-014-2976-8. Epub 2014 Dec 21.
In the present study, we wanted to examine the predominant factor/s in the initiation of metastasis. We used samples of advanced grades of renal clear cell carcinoma with documented clinical history of vena caval spread as the experimental group. The major rationale for this selection is the fact that renal cell carcinoma metastasize extensively through the inferior vena cava up to the pulmonary bed and often exist as a continuous mass of metastatic tissue. As cortactin plays a significant role in invadopodia formation during initiation of metastasis, in the present study, we tested expression of cortactin and phospho(tyr421)-cortactin in different grades of renal cell clear carcinoma and examined its property to bind to actin. The findings of the present study suggest that the variations of the local physiological milieu are the driving forces for metastasis by enhancing molecular mechanisms for lamellipodia formation. We conclude that localization of cortactin in cancer cells and interaction between actin and its nucleators are crucial for cancer progression.
在本研究中，我们想要探究转移起始过程中的主要因素。我们使用具有记录在案的腔静脉转移临床病史的高级别肾透明细胞癌样本作为实验组。做出这种选择的主要理由是，肾细胞癌广泛地通过下腔静脉转移至肺床，并且常常以连续的转移组织团块形式存在。由于皮质肌动蛋白在转移起始过程中的侵袭性伪足形成中发挥重要作用，在本研究中，我们检测了不同级别肾透明细胞癌中皮质肌动蛋白和磷酸化（酪氨酸421）-皮质肌动蛋白的表达，并研究了其与肌动蛋白结合的特性。本研究结果表明，局部生理环境的变化通过增强片状伪足形成的分子机制而成为转移的驱动力。我们得出结论，癌细胞中皮质肌动蛋白的定位以及肌动蛋白与其成核因子之间的相互作用对于癌症进展至关重要。
