Department of Nephro-urology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Urology. 2011 Aug;78(2):476.e9-15. doi: 10.1016/j.urology.2011.03.019. Epub 2011 Jun 21.
To clarify the clinical and prognostic significance of cortactin and phosphorylated cortactin in patients with sarcomatoid renal cell carcinoma (SRCC).
We retrospectively reviewed the data from 31 patients with SRCC and 33 with conventional renal cell carcinoma matched for clinicopathologic features. The immunoreactive score for cortactin, pY421 cortactin, and pY466 cortactin were measured using immunohistochemistry. The relationships between each immunoreactive score and the clinicopathologic features and survival were investigated.
The immunoreactive score of p421 cortactin, but not that of cortactin and pY466 cortactin, was significantly greater in SRCC than in conventional renal cell carcinoma (P < .001). The expression of pY421 cortactin in SRCC correlated with the pT stage and metastasis (P < .001). The expression of pY466 cortactin showed a similar trend with pT stage (P = .043) but not with metastasis. Although both of pY421 cortactin and pY466 cortactin were identified as useful predictors for survival in univariate analyses, only pY421 cortactin expression was considered an independent predictor in patients with SRCC (odds ratio 4.53, 95% confidence interval 1.07-19.12, P = .040) in the multivariate analysis model, including pT stage and metastasis.
Our results have demonstrated that phosphorylation of cortactin is a key process in malignant aggressiveness, and its expression is a useful predictor of cause-specific survival and could be a useful potential therapeutic target in patients with SRCC.
阐明黏着斑蛋白及其磷酸化形式在肉瘤样肾细胞癌（SRCC）患者中的临床和预后意义。
我们回顾性分析了 31 例 SRCC 患者和 33 例常规肾细胞癌患者的临床病理资料，这些患者的临床病理特征相匹配。采用免疫组化法检测黏着斑蛋白、磷酸化黏着斑蛋白（pY421 黏着斑蛋白）和磷酸化黏着斑蛋白（pY466 黏着斑蛋白）的免疫反应性评分。分析每种免疫反应性评分与临床病理特征和生存的关系。
SRCC 中 p421 黏着斑蛋白的免疫反应性评分显著高于常规肾细胞癌（P<0.001），但黏着斑蛋白和 pY466 黏着斑蛋白的免疫反应性评分无显著差异。SRCC 中 pY421 黏着斑蛋白的表达与 pT 分期和转移相关（P<0.001）。pY466 黏着斑蛋白的表达与 pT 分期呈相似趋势（P=0.043），但与转移无关。尽管 pY421 黏着斑蛋白和 pY466 黏着斑蛋白在单因素分析中均被确定为生存的有用预测因子，但在包括 pT 分期和转移的多因素分析模型中，仅 pY421 黏着斑蛋白的表达被认为是 SRCC 患者的独立预测因子（优势比 4.53，95%置信区间 1.07-19.12，P=0.040）。
我们的研究结果表明，黏着斑蛋白的磷酸化是恶性侵袭性的关键过程，其表达是一种有用的生存预测因子，可能成为 SRCC 患者潜在的治疗靶点。
